Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts

Andrew Paul Desbois; Valerie Jane Smith

doi:10.1007/978-1-4939-2684-8_25

Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts

Andrew Paul Desbois, Valerie Jane Smith

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Citations (Scopus)

Abstract

Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches.

Original language	English
Title of host publication	Natural Products From Marine Algae
Place of Publication	Clifton New Jersey USA
Publisher	Springer
Pages	403-410
Number of pages	8
ISBN (Electronic)	9781493926848
ISBN (Print)	9781493926831
DOIs	https://doi.org/10.1007/978-1-4939-2684-8_25
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology
Volume	1308

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-2684-8_25

Cite this

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abstract = "Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches.",

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AU - Smith, Valerie Jane

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AB - Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches.

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ER -

Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts

Abstract

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UN SDGs

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