Direct observation of DNA distortion by the RSC complex

G Lia, E Praly, H Ferreira, C Stockdale, YC Tse-Dinh, D Dunlap, V Croquette, D Bensimon, T Owen-Hughes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

125 Citations (Scopus)

Abstract

The Snf2 family represents a functionally diverse class of ATPase sharing the ability to modify DNA structure. Here, we use a magnetic trap and an atomic force microscope to monitor the activity of a member of this class: the RSC complex. This enzyme caused transient shortenings in DNA length involving translocation of typically 400 bp within 2 s, resulting in the formation of a loop whose size depended on both the force applied to the DNA and the ATP concentration. The majority of loops then decrease in size within a time similar to that with which they are formed, suggesting that the motor has the ability to reverse its direction. Loop formation was also associated with the generation of negative DNA supercoils. These observations support the idea that the ATPase motors of the Snf2 family of proteins act as DNA translocases specialized to generate transient distortions in DNA structure.

Original languageEnglish
Pages (from-to)417-425
Number of pages9
JournalMolecular Cell
Volume21
Issue number3
DOIs
Publication statusPublished - 3 Feb 2006

Keywords

  • CHROMATIN-REMODELING COMPLEX
  • SINGLE-MOLECULE
  • SUPERCOILED DNA
  • TRANSLOCATION
  • ENZYME
  • POLYMERASE
  • FTSK

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