Direct analysis of the secretions of the potato cyst nematode Globodera rostochiensis

L Robertson, WM Robertson, JT Jones*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Secretions were induced from second (invasive) stage juveniles (J2s) of the potato cyst nematode Globodera rostochiensis by exposing them to 5-methoxy-N,N-dimethyl tryptamine oxalate (DMT). Secretions were collected from J2s in sufficient quantity to allow direct analysis. Gel electrophoresis followed by monochromatic silver staining demonstrated the presence of at least 10 proteins. The presence of several enzymes, including superoxide dismutase and proteases, was demonstrated using Western blots and activity assays. Antisera raised against the secretions recognized bands on Western blots consistent in molecular mass with those identified on silver stained gels. The antisera recognized structures implicated in the production of secretions including the subventral gland cells and surface-of J2s.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalParasitology
Volume119
Publication statusPublished - Aug 1999

Keywords

  • Globodera rostochiensis
  • secretions
  • cellulase
  • superoxide dismutase
  • protease
  • antisera
  • PLANT-PARASITIC NEMATODES
  • MONOCLONAL-ANTIBODIES
  • HETERODERA-GLYCINES
  • MELOIDOGYNE-INCOGNITA
  • ARABIDOPSIS-THALIANA
  • FEEDING STRUCTURES
  • STYLET SECRETIONS
  • ESOPHAGEAL GLANDS
  • GENE-EXPRESSION
  • ANTIGENS

Fingerprint

Dive into the research topics of 'Direct analysis of the secretions of the potato cyst nematode Globodera rostochiensis'. Together they form a unique fingerprint.

Cite this