Diagnosis of Streptococcus pneumoniae pneumonia by quantitative enzyme linked immunosorbent assay of C-polysaccharide antigen

S. H. Gillespie*, A. Dickens, J. G. Raynes, K. P.W.J. McAdam

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Aims-To evaluate the use of a quantitative enzyme linked immunosorbent assay (ELISA) detecting C-polysaccharide (PnC) antigen in sputum for the diagnosis of Streptococcus pneumoniae infection. Methods-Specimens of sputum from 60 patients with acute community and hospital acquired pneumonia and infective exacerbations of obstructive airways disease were examined by semiquantitative culture and antigen ELISA. Results-Using a cutoff value of 1 μg/ ml PnC antigen for a positive result, the sensitivity of this assay was 90-3%, specificity 93*1%, predictive value of a positive result was 93-5%, and the predictive value of a negative result 89*6%. Conclusions-Quantitation of C-polysaccharide antigen in sputum by ELISA distinguishes between carriage of oral bacteria which express PnC-like antigen and infection with S pneumoniae and compares favourably with other diagnostic methods.

Original languageEnglish
Pages (from-to)749-751
Number of pages3
JournalJournal of Clinical Pathology
Volume47
Issue number8
DOIs
Publication statusPublished - 1 Jan 1994

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