Development of the magnetic capture system for recovery of Xiphinema americanum

Q Chen, DJF Brown, RHC Curtis, JT Jones*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Xiphinema americanum is listed as a quarantine nematode by The European Plant Protection Organisation because of its ability to transmit quarantine viruses. Detection of this nematode in soil samples, or in mixed nematode samples extracted from soil, is therefore of paramount importance. We recently described the use of magnetic beads (Dynabeads) in combination with probes such as lectins or antibodies for recovery of small endoparasitic nematodes including Meloidogyne spp. and Globodera rostochiensis from mixed nematode samples. Here we show that magnetic capture can be used with much larger nematodes such as X americanum. We describe lectins and antisera that bind to the surface of this nematode and show that the antisera can be used in the Dynabeads system to recover and enrich X americanum from mixed nematode samples.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalAnnals of Applied Biology
Volume143
Issue number3
Publication statusPublished - 2003

Keywords

  • Xiphinema americanum
  • Dynabeads
  • antiserum
  • lectins
  • surface labelling
  • PLANT-PARASITIC NEMATODES
  • SURFACE-COAT
  • BODY WALL
  • IDENTIFICATION
  • RESIDUES

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