Development of nuclear and plastid SNP markers for genetic studies of Dipteryx tree species in Amazonia

Eurídice N. Honorio Coronado*, Céline Blanc-Jolivet, Malte Mader, Carmen R. García-Dávila, Alexandre M. Sebbenn, Barbara R.V. Meyer-Sand, Kathelyn Paredes-Villanueva, Niklas Tysklind, Valerie Troispoux, Marie Massot, Bernd Degen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We developed nuclear and plastid single nucleotide polymorphism (SNP) and insertion/deletion (INDEL) markers for Dipteryx species using a combination of restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing. Of the total 315 loci genotyped using a MassARRAY platform, 292 loci were variable and polymorphic among the 73 sampled individuals from French Guiana, Brasil, Peru, and Bolivia. A final set of 56 nuclear SNPs, 26 chloroplast SNPs, 2 chloroplast INDELs, and 32 mitochondrial SNPs identifying significant population structure was developed. This set of loci will be useful for studies on population genetics of Dipteryx species in Amazonia.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalConservation Genetics Resources
Volume11
Issue number3
DOIs
Publication statusPublished - 15 Sept 2019

Keywords

  • Cumaru
  • MassARRAY
  • SNP
  • Timber species

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