Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

Jonathan Nylk, M. V. G. Kristensen, Michael Mazilu, Anisha Kubasik-Thayil, C. A. Mitchell, Elaine Catherine Campbell, Simon John Powis, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
6 Downloads (Pure)

Abstract

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
Original languageEnglish
Pages (from-to)1512-1519
JournalBiomedical Optics Express
Volume6
Issue number4
Early online date27 Mar 2015
DOIs
Publication statusPublished - 1 Apr 2015

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