Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

Jonathan Nylk; M. V. G. Kristensen; Michael Mazilu; Anisha Kubasik-Thayil; C. A. Mitchell; Elaine Catherine Campbell; Simon John Powis; Frank J Gunn-Moore; Kishan Dholakia

doi:10.1364/BOE.6.001512

Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

Jonathan Nylk, M. V. G. Kristensen, Michael Mazilu, Anisha Kubasik-Thayil, C. A. Mitchell, Elaine Catherine Campbell, Simon John Powis, Frank J Gunn-Moore, Kishan Dholakia

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Abstract

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

Original language	English
Pages (from-to)	1512-1519
Journal	Biomedical Optics Express
Volume	6
Issue number	4
Early online date	27 Mar 2015
DOIs	https://doi.org/10.1364/BOE.6.001512
Publication status	Published - 1 Apr 2015

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Nylk_2015_BOE_Dev_AM
© 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited
Accepted author manuscript, 593 KB

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light
Dholakia, K. (PI), Krauss, T. F. (CoI) & Samuel, I. D. W. (CoI)
EPSRC
1/06/12 → 31/05/17
Project: Standard

Data underpinning - Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis
Nylk, J. (Creator), Kristensen, M. V. G. (Creator), Mazilu, M. (Creator), Kubasik-Thayil, A. (Creator), Mitchell, C. A. (Creator), Campbell, E. C. (Creator), Powis, S. J. (Creator), Gunn-Moore, F. J. (Creator) & Dholakia, K. (Creator), University of St Andrews, 2015
DOI: 10.17630/68a91fdb-11f2-4410-8f2e-dd436b016bc7
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title = "Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis",

abstract = "We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.",

author = "Jonathan Nylk and Kristensen, {M. V. G.} and Michael Mazilu and Anisha Kubasik-Thayil and Mitchell, {C. A.} and Campbell, {Elaine Catherine} and Powis, {Simon John} and Gunn-Moore, {Frank J} and Kishan Dholakia",

note = "The authors thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1. ",

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doi = "10.1364/BOE.6.001512",

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T1 - Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

AU - Nylk, Jonathan

AU - Kristensen, M. V. G.

AU - Mazilu, Michael

AU - Kubasik-Thayil, Anisha

AU - Mitchell, C. A.

AU - Campbell, Elaine Catherine

AU - Powis, Simon John

AU - Gunn-Moore, Frank J

AU - Dholakia, Kishan

N1 - The authors thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

AB - We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

U2 - 10.1364/BOE.6.001512

DO - 10.1364/BOE.6.001512

M3 - Article

SN - 2156-7085

VL - 6

SP - 1512

EP - 1519

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 4

ER -

Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

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Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

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Data underpinning - Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

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