Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies

Marcella Orwick-Rydmark; Janet E. Lovett; Andrea Graziadei; Ljubica Lindholm; Matthew R. Hicks; Anthony Watts

doi:10.1021/nl3020395

Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies

Marcella Orwick-Rydmark, Janet E. Lovett, Andrea Graziadei, Ljubica Lindholm, Matthew R. Hicks, Anthony Watts^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.

Original language	English
Pages (from-to)	4687-4692
Number of pages	6
Journal	Nano Letters
Volume	12
Issue number	9
DOIs	https://doi.org/10.1021/nl3020395
Publication status	Published - Sept 2012

Keywords

Lipodisq
bilayer
electron paramagnetic resonance
double electron electron resonance
bacteriorhodopsin
photoreceptor
ELECTRON DOUBLE-RESONANCE
LIPIDIC CUBIC PHASES
MEMBRANE-PROTEINS
DISTANCE MEASUREMENTS
2-DIMENSIONAL CRYSTALS
HALOBACTERIUM-HALOBIUM
PURPLE MEMBRANE
SIDE-CHAINS
SPIN-LABEL
BACTERIORHODOPSIN

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10.1021/nl3020395

Royal Society Research Fellowship: Royal Society Research Fellowship
Lovett, J. E. (PI)
The Royal Society
1/05/14 → 15/02/17
Project: Fellowship

Cite this

@article{b03191ff06a94645b74f091272176620,

title = "Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies",

abstract = "SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.",

keywords = "Lipodisq, bilayer, electron paramagnetic resonance, double electron electron resonance, bacteriorhodopsin, photoreceptor, ELECTRON DOUBLE-RESONANCE, LIPIDIC CUBIC PHASES, MEMBRANE-PROTEINS, DISTANCE MEASUREMENTS, 2-DIMENSIONAL CRYSTALS, HALOBACTERIUM-HALOBIUM, PURPLE MEMBRANE, SIDE-CHAINS, SPIN-LABEL, BACTERIORHODOPSIN",

author = "Marcella Orwick-Rydmark and Lovett, {Janet E.} and Andrea Graziadei and Ljubica Lindholm and Hicks, {Matthew R.} and Anthony Watts",

year = "2012",

month = sep,

doi = "10.1021/nl3020395",

language = "English",

volume = "12",

pages = "4687--4692",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society (ACS)",

number = "9",

}

TY - JOUR

T1 - Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies

AU - Orwick-Rydmark, Marcella

AU - Lovett, Janet E.

AU - Graziadei, Andrea

AU - Lindholm, Ljubica

AU - Hicks, Matthew R.

AU - Watts, Anthony

PY - 2012/9

Y1 - 2012/9

N2 - SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.

AB - SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.

KW - Lipodisq

KW - bilayer

KW - electron paramagnetic resonance

KW - double electron electron resonance

KW - bacteriorhodopsin

KW - photoreceptor

KW - ELECTRON DOUBLE-RESONANCE

KW - LIPIDIC CUBIC PHASES

KW - MEMBRANE-PROTEINS

KW - DISTANCE MEASUREMENTS

KW - 2-DIMENSIONAL CRYSTALS

KW - HALOBACTERIUM-HALOBIUM

KW - PURPLE MEMBRANE

KW - SIDE-CHAINS

KW - SPIN-LABEL

KW - BACTERIORHODOPSIN

U2 - 10.1021/nl3020395

DO - 10.1021/nl3020395

M3 - Article

SN - 1530-6984

VL - 12

SP - 4687

EP - 4692

JO - Nano Letters

JF - Nano Letters

IS - 9

ER -

Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies

Abstract

Keywords

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Projects

Royal Society Research Fellowship: Royal Society Research Fellowship

Cite this