Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies

Marcella Orwick-Rydmark, Janet E. Lovett, Andrea Graziadei, Ljubica Lindholm, Matthew R. Hicks, Anthony Watts*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)

Abstract

SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.

Original languageEnglish
Pages (from-to)4687-4692
Number of pages6
JournalNano Letters
Volume12
Issue number9
DOIs
Publication statusPublished - Sept 2012

Keywords

  • Lipodisq
  • bilayer
  • electron paramagnetic resonance
  • double electron electron resonance
  • bacteriorhodopsin
  • photoreceptor
  • ELECTRON DOUBLE-RESONANCE
  • LIPIDIC CUBIC PHASES
  • MEMBRANE-PROTEINS
  • DISTANCE MEASUREMENTS
  • 2-DIMENSIONAL CRYSTALS
  • HALOBACTERIUM-HALOBIUM
  • PURPLE MEMBRANE
  • SIDE-CHAINS
  • SPIN-LABEL
  • BACTERIORHODOPSIN

Fingerprint

Dive into the research topics of 'Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies'. Together they form a unique fingerprint.

Cite this