Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O Baron; Mingzhou Chen; Simon O Clark; Ann Williams; Kishan Dholakia; Stephen H Gillespie

doi:10.1007/978-1-4939-7638-6_4

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O Baron, Mingzhou Chen, Simon O Clark, Ann Williams, Kishan Dholakia, Stephen H Gillespie

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

Original language	English
Title of host publication	Antibiotic Resistance Protocols
Editors	Stephen Gillespie
Place of Publication	New York, NY
Publisher	Humana Press/Springer
Pages	41-50
Number of pages	10
ISBN (Electronic)	9781493976386
ISBN (Print)	9781493976362
DOIs	https://doi.org/10.1007/978-1-4939-7638-6_4
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	1736
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Raman spectroscopy
Mycobacteria
Phenotypic resistance
Lipids

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10.1007/978-1-4939-7638-6_4

Baron-2018-Detecting-phenotypically-ARP-AAM
© 2018, Springer Science+Business Media, LLC. This work has been made available online by kind permission of the publisher. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1007/978-1-4939-7638-6_4
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Baron, V. O., Chen, M., Clark, S. O., Williams, A., Dholakia, K., & Gillespie, S. H. (2018). Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. In S. Gillespie (Ed.), Antibiotic Resistance Protocols (pp. 41-50). (Methods in Molecular Biology; Vol. 1736). Humana Press/Springer. https://doi.org/10.1007/978-1-4939-7638-6_4

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title = "Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy",

abstract = "Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.",

keywords = "Raman spectroscopy, Mycobacteria, Phenotypic resistance, Lipids",

author = "Baron, \{Vincent O\} and Mingzhou Chen and Clark, \{Simon O\} and Ann Williams and Kishan Dholakia and Gillespie, \{Stephen H\}",

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language = "English",

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Baron, VO, Chen, M, Clark, SO, Williams, A, Dholakia, K & Gillespie, SH 2018, Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. in S Gillespie (ed.), Antibiotic Resistance Protocols. Methods in Molecular Biology, vol. 1736, Humana Press/Springer, New York, NY, pp. 41-50. https://doi.org/10.1007/978-1-4939-7638-6_4

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. / Baron, Vincent O; Chen, Mingzhou; Clark, Simon O et al.
Antibiotic Resistance Protocols. ed. / Stephen Gillespie. New York, NY: Humana Press/Springer, 2018. p. 41-50 (Methods in Molecular Biology; Vol. 1736).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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T1 - Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

AU - Baron, Vincent O

AU - Chen, Mingzhou

AU - Clark, Simon O

AU - Williams, Ann

AU - Dholakia, Kishan

AU - Gillespie, Stephen H

PY - 2018

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N2 - Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

AB - Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

KW - Raman spectroscopy

KW - Mycobacteria

KW - Phenotypic resistance

KW - Lipids

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T3 - Methods in Molecular Biology

SP - 41

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BT - Antibiotic Resistance Protocols

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PB - Humana Press/Springer

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ER -

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Abstract

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