Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

Mingzhou Chen; Josep Mas; Lindsey H. Forbes; Melissa R. Andrews; Kishan Dholakia

doi:10.1002/jbio.201700129

Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

Mingzhou Chen, Josep Mas, Lindsey H. Forbes, Melissa R. Andrews, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.
Our approach is also capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter within a brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.

Original language	English
Article number	e201700129
Number of pages	7
Journal	Journal of Biophotonics
Volume	11
Issue number	1
Early online date	17 Aug 2017
DOIs	https://doi.org/10.1002/jbio.201700129
Publication status	Published - Jan 2018

Keywords

Raman spectroscopy
Optical coherence tomography
Turbid media
Brain tissue

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10.1002/jbio.201700129

wmrs_v1
© 2017, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at onlinelibrary.wiley.com / https://doi.org/10.1002/jbio.201700129
Accepted author manuscript, 2.35 MB

Shaped light at the interface: Shaped Light at the Interface
Dholakia, K. (PI), Brown, C. T. A. (CoI), Di Falco, A. (CoI) & Mazilu, M. (CoI)
EPSRC
1/09/14 → 31/08/19
Project: Standard
EU FP7 FAMOS: Functional Anatomical Molecular Optical Screening
Dholakia, K. (PI)
European Commission
1/10/12 → 31/03/17
Project: Standard
Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light
Dholakia, K. (PI), Krauss, T. F. (CoI) & Samuel, I. D. W. (CoI)
EPSRC
1/06/12 → 31/05/17
Project: Standard

Data Underpinning: "Depth-resolved multimodal imaging: Wavelength Modulated Spatially Offset Raman Spectroscopy with Optical Coherence Tomography"
Chen, M. (Creator), Mas Soler, J. (Creator), Forbes, L. H. (Creator), Andrews, M. R. (Creator) & Dholakia, K. (Creator), University of St Andrews, 14 Jul 2016
DOI: 10.17630/4f1b2967-4c82-44be-a179-b6e28afd328e
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Cite this

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title = "Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography",

abstract = "A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.Our approach is also capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter within a brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.",

keywords = "Raman spectroscopy, Optical coherence tomography, Turbid media, Brain tissue",

author = "Mingzhou Chen and Josep Mas and Forbes, {Lindsey H.} and Andrews, {Melissa R.} and Kishan Dholakia",

note = "Funding: UK Engineering and Physical Sciences Research Council (EPSRC: EP/J01771X/1, EP/M000869/1), the European Union FAMOS project (FP7 ICT, 317744) and the RS MacDonald Charitable Trust for funding.",

year = "2018",

month = jan,

doi = "10.1002/jbio.201700129",

language = "English",

volume = "11",

journal = "Journal of Biophotonics",

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TY - JOUR

T1 - Depth-resolved multimodal imaging

T2 - wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

AU - Chen, Mingzhou

AU - Mas, Josep

AU - Forbes, Lindsey H.

AU - Andrews, Melissa R.

AU - Dholakia, Kishan

N1 - Funding: UK Engineering and Physical Sciences Research Council (EPSRC: EP/J01771X/1, EP/M000869/1), the European Union FAMOS project (FP7 ICT, 317744) and the RS MacDonald Charitable Trust for funding.

PY - 2018/1

Y1 - 2018/1

N2 - A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.Our approach is also capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter within a brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.

AB - A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.Our approach is also capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter within a brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.

KW - Raman spectroscopy

KW - Optical coherence tomography

KW - Turbid media

KW - Brain tissue

U2 - 10.1002/jbio.201700129

DO - 10.1002/jbio.201700129

M3 - Article

SN - 1864-063X

VL - 11

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 1

M1 - e201700129

ER -

Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

Abstract

Keywords

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Projects

Shaped light at the interface: Shaped Light at the Interface

EU FP7 FAMOS: Functional Anatomical Molecular Optical Screening

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

Datasets

Data Underpinning: "Depth-resolved multimodal imaging: Wavelength Modulated Spatially Offset Raman Spectroscopy with Optical Coherence Tomography"

Cite this