Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

Mingzhou Chen, Josep Mas, Lindsey H. Forbes, Melissa R. Andrews, Kishan Dholakia

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26 Citations (Scopus)
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Abstract

A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth co-localization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.
Our approach is also capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter within a brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.
Original languageEnglish
Article numbere201700129
Number of pages7
JournalJournal of Biophotonics
Volume11
Issue number1
Early online date17 Aug 2017
DOIs
Publication statusPublished - Jan 2018

Keywords

  • Raman spectroscopy
  • Optical coherence tomography
  • Turbid media
  • Brain tissue

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