Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma

R Madan; M Brandwein-Gensler; NF Schlecht; K Elias; E Gorbovitsky; TJ Belbin; R Mahood; D Breining; H Qian; G Childs; J Locker; R Smith; M Haigentz Jr; Francis James Gunn-Moore; MB Prystowsky

doi:10.1002/hed.20435

Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma

R Madan, M Brandwein-Gensler, NF Schlecht, K Elias, E Gorbovitsky, TJ Belbin, R Mahood, D Breining, H Qian, G Childs, J Locker, R Smith, M Haigentz Jr, Francis James Gunn-Moore, MB Prystowsky

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC).

Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue, TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables.

Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (> 92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p=.04, hazard ratio 1.82)

Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome. (c) 2006 Wiley Periodicals, Inc.

Original language	English
Pages (from-to)	1018-1027
Number of pages	10
Journal	Head and Neck
Volume	28(11)
DOIs	https://doi.org/10.1002/hed.20435
Publication status	Published - Nov 2006

Keywords

head and neck neoplasms
immunohistochemistry
ezrin
moesin
survival analysis
NF2 TUMOR-SUPPRESSOR
GENE-EXPRESSION
MOLECULAR CLASSIFICATION
MICROARRAY ANALYSIS
CDNA MICROARRAYS
CANCER
MOESIN
IDENTIFICATION
MERLIN
METASTASIS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/hed.20435

Cite this

Madan, R., Brandwein-Gensler, M., Schlecht, NF., Elias, K., Gorbovitsky, E., Belbin, TJ., Mahood, R., Breining, D., Qian, H., Childs, G., Locker, J., Smith, R., Haigentz Jr, M., Gunn-Moore, F. J., & Prystowsky, MB. (2006). Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma. Head and Neck, 28(11), 1018-1027. https://doi.org/10.1002/hed.20435

@article{4f7afc8f4f0744249948da71175669a2,

title = "Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma",

abstract = "Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC).Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue, TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables.Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (> 92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p=.04, hazard ratio 1.82)Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome. (c) 2006 Wiley Periodicals, Inc.",

keywords = "head and neck neoplasms, immunohistochemistry, ezrin, moesin, survival analysis, NF2 TUMOR-SUPPRESSOR, GENE-EXPRESSION, MOLECULAR CLASSIFICATION, MICROARRAY ANALYSIS, CDNA MICROARRAYS, CANCER, MOESIN, IDENTIFICATION, MERLIN, METASTASIS",

author = "R Madan and M Brandwein-Gensler and NF Schlecht and K Elias and E Gorbovitsky and TJ Belbin and R Mahood and D Breining and H Qian and G Childs and J Locker and R Smith and {Haigentz Jr}, M and Gunn-Moore, {Francis James} and MB Prystowsky",

year = "2006",

month = nov,

doi = "10.1002/hed.20435",

language = "English",

volume = "28(11)",

pages = "1018--1027",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "John Wiley & Sons, Ltd",

}

Madan, R, Brandwein-Gensler, M, Schlecht, NF, Elias, K, Gorbovitsky, E, Belbin, TJ, Mahood, R, Breining, D, Qian, H, Childs, G, Locker, J, Smith, R, Haigentz Jr, M, Gunn-Moore, FJ & Prystowsky, MB 2006, 'Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma', Head and Neck, vol. 28(11), pp. 1018-1027. https://doi.org/10.1002/hed.20435

TY - JOUR

T1 - Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma

AU - Madan, R

AU - Brandwein-Gensler, M

AU - Schlecht, NF

AU - Elias, K

AU - Gorbovitsky, E

AU - Belbin, TJ

AU - Mahood, R

AU - Breining, D

AU - Qian, H

AU - Childs, G

AU - Locker, J

AU - Smith, R

AU - Haigentz Jr, M

AU - Gunn-Moore, Francis James

AU - Prystowsky, MB

PY - 2006/11

Y1 - 2006/11

N2 - Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC).Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue, TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables.Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (> 92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p=.04, hazard ratio 1.82)Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome. (c) 2006 Wiley Periodicals, Inc.

AB - Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC).Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue, TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables.Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (> 92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p=.04, hazard ratio 1.82)Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome. (c) 2006 Wiley Periodicals, Inc.

KW - head and neck neoplasms

KW - immunohistochemistry

KW - ezrin

KW - moesin

KW - survival analysis

KW - NF2 TUMOR-SUPPRESSOR

KW - GENE-EXPRESSION

KW - MOLECULAR CLASSIFICATION

KW - MICROARRAY ANALYSIS

KW - CDNA MICROARRAYS

KW - CANCER

KW - MOESIN

KW - IDENTIFICATION

KW - MERLIN

KW - METASTASIS

UR - http://www.scopus.com/inward/record.url?scp=33750375071&partnerID=8YFLogxK

U2 - 10.1002/hed.20435

DO - 10.1002/hed.20435

M3 - Article

SN - 1043-3074

VL - 28(11)

SP - 1018

EP - 1027

JO - Head and Neck

JF - Head and Neck

ER -

Cytoplasmic ezrin has prognostic significance for head and neck squamous cell carcinoma

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this