Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph

John Langford Wray; JR Howarth; MA Roberts

Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph

John Langford Wray, JR Howarth, MA Roberts

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	Biochimica et Biophysica Acta
Volume	1350
Publication status	Published - 7 Feb 1997

Keywords

serine acetyltransferase
cysteine biosynthesis
nucleotide sequence
sulfate regulation
O-ACETYLSERINE(THIOL)LYASE
SULFATE
IDENTIFICATION
CHLOROPLASTS
SULFURYLASE

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title = "Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph",

abstract = "A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.",

keywords = "serine acetyltransferase, cysteine biosynthesis, nucleotide sequence, sulfate regulation, O-ACETYLSERINE(THIOL)LYASE, SULFATE, IDENTIFICATION, CHLOROPLASTS, SULFURYLASE",

author = "Wray, \{John Langford\} and JR Howarth and MA Roberts",

year = "1997",

month = feb,

day = "7",

language = "English",

volume = "1350",

pages = "123--127",

journal = "Biochimica et Biophysica Acta",

issn = "0006-3002",

}

Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph. / Wray, John Langford; Howarth, JR; Roberts, MA.
In: Biochimica et Biophysica Acta, Vol. 1350, 07.02.1997, p. 123-127.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph

AU - Wray, John Langford

AU - Howarth, JR

AU - Roberts, MA

PY - 1997/2/7

Y1 - 1997/2/7

N2 - A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.

AB - A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.

KW - serine acetyltransferase

KW - cysteine biosynthesis

KW - nucleotide sequence

KW - sulfate regulation

KW - O-ACETYLSERINE(THIOL)LYASE

KW - SULFATE

KW - IDENTIFICATION

KW - CHLOROPLASTS

KW - SULFURYLASE

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UR - https://www.scopus.com/pages/publications/0031055355

M3 - Article

SN - 0006-3002

VL - 1350

SP - 123

EP - 127

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

ER -

Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph

Abstract

Keywords

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