Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph

John Langford Wray, JR Howarth, MA Roberts

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalBiochimica et Biophysica Acta
Volume1350
Publication statusPublished - 7 Feb 1997

Keywords

  • serine acetyltransferase
  • cysteine biosynthesis
  • nucleotide sequence
  • sulfate regulation
  • O-ACETYLSERINE(THIOL)LYASE
  • SULFATE
  • IDENTIFICATION
  • CHLOROPLASTS
  • SULFURYLASE

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