Cyclic-AMP regulation of calcium-dependent K channels in an insect central neurone

JA David; RM Pitman

doi:10.1016/0304-3940(96)12300-4

Cyclic-AMP regulation of calcium-dependent K channels in an insect central neurone

JA David^*, RM Pitman

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

In the cockroach fast coral depressor motoneurone, either the muscarinic agonist McN-A-343 or dibutyryl cAMP (Db-cAMP) induced a reduction in voltage-dependent outward current. The response to McN is due to suppression of a calcium-dependent potassium current (I-K,I-Ca) produced secondarily to a reduction in voltage-dependent calcium current (I-Ca). The response to Db-cAMP was investigated in order to establish whether cAMP might mediate the response to McN. I-Ca was suppressed by 3-isobutyl-1-methylranthine (IBMX) but not by Db-cAMP. The effects of IBMX were therefore unlikely to be the result of phosphodiesterase inhibition. Since caffeine also suppressed I-Ca, the observed effect of IBMX is probably due to release of Ca2+ from intracellular stores. I-K,I-Ca evoked by injection of Ca2+, was reduced by Db-cAMP or forskolin but not by McN. These results indicate that the electrical response to McN in this neurone is not mediated by changes in cAMP.

Original language	English
Pages (from-to)	151-154
Number of pages	4
Journal	Neuroscience Letters
Volume	203
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(96)12300-4
Publication status	Published - 26 Jan 1996

Keywords

c-AMP
calcium-dependent potassium currents
muscarinic
insect neurone
phosphoinositides
3-isobutyl-1-methylxanthine
caffeine
MUSCARINIC ACETYLCHOLINE-RECEPTOR
MOLECULAR-BASIS

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10.1016/0304-3940(96)12300-4

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title = "Cyclic-AMP regulation of calcium-dependent K channels in an insect central neurone",

abstract = "In the cockroach fast coral depressor motoneurone, either the muscarinic agonist McN-A-343 or dibutyryl cAMP (Db-cAMP) induced a reduction in voltage-dependent outward current. The response to McN is due to suppression of a calcium-dependent potassium current (I-K,I-Ca) produced secondarily to a reduction in voltage-dependent calcium current (I-Ca). The response to Db-cAMP was investigated in order to establish whether cAMP might mediate the response to McN. I-Ca was suppressed by 3-isobutyl-1-methylranthine (IBMX) but not by Db-cAMP. The effects of IBMX were therefore unlikely to be the result of phosphodiesterase inhibition. Since caffeine also suppressed I-Ca, the observed effect of IBMX is probably due to release of Ca2+ from intracellular stores. I-K,I-Ca evoked by injection of Ca2+, was reduced by Db-cAMP or forskolin but not by McN. These results indicate that the electrical response to McN in this neurone is not mediated by changes in cAMP.",

keywords = "c-AMP, calcium-dependent potassium currents, muscarinic, insect neurone, phosphoinositides, 3-isobutyl-1-methylxanthine, caffeine, MUSCARINIC ACETYLCHOLINE-RECEPTOR, MOLECULAR-BASIS",

author = "JA David and RM Pitman",

year = "1996",

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doi = "10.1016/0304-3940(96)12300-4",

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AU - David, JA

AU - Pitman, RM

PY - 1996/1/26

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N2 - In the cockroach fast coral depressor motoneurone, either the muscarinic agonist McN-A-343 or dibutyryl cAMP (Db-cAMP) induced a reduction in voltage-dependent outward current. The response to McN is due to suppression of a calcium-dependent potassium current (I-K,I-Ca) produced secondarily to a reduction in voltage-dependent calcium current (I-Ca). The response to Db-cAMP was investigated in order to establish whether cAMP might mediate the response to McN. I-Ca was suppressed by 3-isobutyl-1-methylranthine (IBMX) but not by Db-cAMP. The effects of IBMX were therefore unlikely to be the result of phosphodiesterase inhibition. Since caffeine also suppressed I-Ca, the observed effect of IBMX is probably due to release of Ca2+ from intracellular stores. I-K,I-Ca evoked by injection of Ca2+, was reduced by Db-cAMP or forskolin but not by McN. These results indicate that the electrical response to McN in this neurone is not mediated by changes in cAMP.

AB - In the cockroach fast coral depressor motoneurone, either the muscarinic agonist McN-A-343 or dibutyryl cAMP (Db-cAMP) induced a reduction in voltage-dependent outward current. The response to McN is due to suppression of a calcium-dependent potassium current (I-K,I-Ca) produced secondarily to a reduction in voltage-dependent calcium current (I-Ca). The response to Db-cAMP was investigated in order to establish whether cAMP might mediate the response to McN. I-Ca was suppressed by 3-isobutyl-1-methylranthine (IBMX) but not by Db-cAMP. The effects of IBMX were therefore unlikely to be the result of phosphodiesterase inhibition. Since caffeine also suppressed I-Ca, the observed effect of IBMX is probably due to release of Ca2+ from intracellular stores. I-K,I-Ca evoked by injection of Ca2+, was reduced by Db-cAMP or forskolin but not by McN. These results indicate that the electrical response to McN in this neurone is not mediated by changes in cAMP.

KW - c-AMP

KW - calcium-dependent potassium currents

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KW - insect neurone

KW - phosphoinositides

KW - 3-isobutyl-1-methylxanthine

KW - caffeine

KW - MUSCARINIC ACETYLCHOLINE-RECEPTOR

KW - MOLECULAR-BASIS

U2 - 10.1016/0304-3940(96)12300-4

DO - 10.1016/0304-3940(96)12300-4

M3 - Article

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VL - 203

SP - 151

EP - 154

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Cyclic-AMP regulation of calcium-dependent K channels in an insect central neurone

Abstract

Keywords

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