Crystallization of Ranasmurfin, a blue coloured protein from Polypedates leucomystax

Stephen McMahon, MA Walsh, RTY Ching, Lester Carter, M Dorward, Kenneth Alan Johnson, Huanting Liu, Muse Oke, C Block Jr, MW Kennedy, AA Latiff, A Cooper, Garry Lindsay Taylor, Malcolm Frederick White, James Henderson Naismith

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8 Citations (Scopus)

Abstract

Ranasmurfin, a previously uncharacterized similar to 13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 angstrom with P2(1) symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 angstrom, beta = 93.3 degrees. Self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.

Original languageEnglish
Pages (from-to)1124-1126
Number of pages3
JournalActa Crystallographica. Section F, Structural biology and crystallization communications
Volume62
Issue numberPt 11
DOIs
Publication statusPublished - Nov 2006

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  • BBSRC BBS/B/14426: SPORT

    Naismith, J.

    BBSRC

    18/10/0430/04/12

    Project: Standard

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