Abstract
A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=94.6, b=215.5, c=218.5 Angstrom. A data set to 3.0 Angstrom with 99.8% completeness and an R-merge of 10.5% has been collected from a single crystal.
| Original language | English |
|---|---|
| Pages (from-to) | 558-560 |
| Number of pages | 3 |
| Journal | Acta Crystallographica. Section D, Biological crystallography |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2004 |
Keywords
- EXPRESSION
- SURFACE
- COMPLEX
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