TY - JOUR
T1 - Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30
AU - Beis, K
AU - Nesper, J
AU - Whitfield, C
AU - Naismith, James Henderson
PY - 2004/3
Y1 - 2004/3
N2 - A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=94.6, b=215.5, c=218.5 Angstrom. A data set to 3.0 Angstrom with 99.8% completeness and an R-merge of 10.5% has been collected from a single crystal.
AB - A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=94.6, b=215.5, c=218.5 Angstrom. A data set to 3.0 Angstrom with 99.8% completeness and an R-merge of 10.5% has been collected from a single crystal.
KW - EXPRESSION
KW - SURFACE
KW - COMPLEX
UR - http://www.scopus.com/inward/record.url?scp=7444249547&partnerID=8YFLogxK
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14993692&itool=iconabstr
U2 - 10.1107/S0907444903029494
DO - 10.1107/S0907444903029494
M3 - Article
SN - 0907-4449
VL - 60
SP - 558
EP - 560
JO - Acta Crystallographica. Section D, Biological crystallography
JF - Acta Crystallographica. Section D, Biological crystallography
IS - 3
ER -