CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF RECOMBINANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM APPLE - A KEY ENZYME IN THE BIOSYNTHESIS OF THE PLANT HORMONE ETHYLENE

E  HOHENESTER; Malcolm F White; J F  KIRSCH; J N  JANSONIUS

CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF RECOMBINANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM APPLE - A KEY ENZYME IN THE BIOSYNTHESIS OF THE PLANT HORMONE ETHYLENE

E HOHENESTER, Malcolm F White, J F KIRSCH, J N JANSONIUS

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

Crystals of recombinant 1-aminocyclopropane-1-carboxylate synthase from apple have been obtained with polyethylene glycol as precipitant using a combination of vapour diffusion and macroseeding techniques. The crystals are of space group P2(1), with unit-cell constants a = 53.7 Angstrom, b = 69.3 Angstrom, c = 123.7 Angstrom and beta = 89.9 degrees. The asymmetric unit content is a 1-aminocyclopropane-1-carboxylate synthase dimer with a molecular mass of 94 kDa. Diffraction extends to 2.2 Angstrom resolution.

Original language	English
Pages (from-to)	947-949
Number of pages	3
Journal	Journal of Molecular Biology
Volume	243
Issue number	5
Publication status	Published - 11 Nov 1994

Keywords

ETHYLENE BIOSYNTHESIS
PYRIDOXAL-5'-PHOSPHATE
CRYSTALLIZATION
MACROSEEDING
X-RAY CRYSTALLOGRAPHY
PROTEINS
FRUIT

Cite this

@article{8858e82f3c704a129bb29880e4060ab4,

title = "CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF RECOMBINANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM APPLE - A KEY ENZYME IN THE BIOSYNTHESIS OF THE PLANT HORMONE ETHYLENE",

abstract = "Crystals of recombinant 1-aminocyclopropane-1-carboxylate synthase from apple have been obtained with polyethylene glycol as precipitant using a combination of vapour diffusion and macroseeding techniques. The crystals are of space group P2(1), with unit-cell constants a = 53.7 Angstrom, b = 69.3 Angstrom, c = 123.7 Angstrom and beta = 89.9 degrees. The asymmetric unit content is a 1-aminocyclopropane-1-carboxylate synthase dimer with a molecular mass of 94 kDa. Diffraction extends to 2.2 Angstrom resolution.",

keywords = "ETHYLENE BIOSYNTHESIS, PYRIDOXAL-5'-PHOSPHATE, CRYSTALLIZATION, MACROSEEDING, X-RAY CRYSTALLOGRAPHY, PROTEINS, FRUIT",

author = "E HOHENESTER and White, \{Malcolm F\} and KIRSCH, \{J F\} and JANSONIUS, \{J N\}",

year = "1994",

month = nov,

day = "11",

language = "English",

volume = "243",

pages = "947--949",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press/Elsevier ",

number = "5",

}

CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF RECOMBINANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM APPLE - A KEY ENZYME IN THE BIOSYNTHESIS OF THE PLANT HORMONE ETHYLENE. / HOHENESTER, E ; White, Malcolm F; KIRSCH, J F et al.
In: Journal of Molecular Biology, Vol. 243, No. 5, 11.11.1994, p. 947-949.

Research output: Contribution to journal › Comment/debate › peer-review

TY - JOUR

T1 - CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF RECOMBINANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM APPLE - A KEY ENZYME IN THE BIOSYNTHESIS OF THE PLANT HORMONE ETHYLENE

AU - HOHENESTER, E

AU - White, Malcolm F

AU - KIRSCH, J F

AU - JANSONIUS, J N

PY - 1994/11/11

Y1 - 1994/11/11

N2 - Crystals of recombinant 1-aminocyclopropane-1-carboxylate synthase from apple have been obtained with polyethylene glycol as precipitant using a combination of vapour diffusion and macroseeding techniques. The crystals are of space group P2(1), with unit-cell constants a = 53.7 Angstrom, b = 69.3 Angstrom, c = 123.7 Angstrom and beta = 89.9 degrees. The asymmetric unit content is a 1-aminocyclopropane-1-carboxylate synthase dimer with a molecular mass of 94 kDa. Diffraction extends to 2.2 Angstrom resolution.

AB - Crystals of recombinant 1-aminocyclopropane-1-carboxylate synthase from apple have been obtained with polyethylene glycol as precipitant using a combination of vapour diffusion and macroseeding techniques. The crystals are of space group P2(1), with unit-cell constants a = 53.7 Angstrom, b = 69.3 Angstrom, c = 123.7 Angstrom and beta = 89.9 degrees. The asymmetric unit content is a 1-aminocyclopropane-1-carboxylate synthase dimer with a molecular mass of 94 kDa. Diffraction extends to 2.2 Angstrom resolution.

KW - ETHYLENE BIOSYNTHESIS

KW - PYRIDOXAL-5'-PHOSPHATE

KW - CRYSTALLIZATION

KW - MACROSEEDING

KW - X-RAY CRYSTALLOGRAPHY

KW - PROTEINS

KW - FRUIT

UR - https://www.scopus.com/pages/publications/0027944321

M3 - Comment/debate

SN - 0022-2836

VL - 243

SP - 947

EP - 949

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 5

ER -

CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF RECOMBINANT 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE FROM APPLE - A KEY ENZYME IN THE BIOSYNTHESIS OF THE PLANT HORMONE ETHYLENE

Abstract

Keywords

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