Crystal Structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, Representatives of Two Families of Tyrosine Phosphatases that Regulate Capsule Assembly

Gregor Hagelueken, Hexian Huang, Iain L. Mainprize, Chris Whitfield, James Henderson Naismith

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Many Gram-positive and Gram-negative bacteria utilize polysaccharide surface layers called capsules to evade the immune system; consequently, the synthesis and export of the capsule are a potential therapeutic target. In Escherichia coli K-30, the integral membrane tyrosine autokinase Wzc and the cognate phosphatase Wzb have been shown to be key for both synthesis and assembly of capsular polysaccharides. In the Gram-positive bacterium Streptococcus pneumoniae, the CpsCD complex is analogous to Wzc and the phosphatase CpsB is the corresponding cognate phosphatase. The phosphatases are known to dephosphorylate their corresponding auto-kinases, yet despite their functional equivalence, they share no sequence homology. We present the structure of Wzb in complex with phosphate and high-resolution structures of apo-CpsB and a phosphate-complexed CpsB. We show that both proteins are active toward Wzc and thereby demonstrate that CpsB is not specific for CpsCD. CpsB is a novel enzyme and represents the first solved structure of a tyrosine phosphatase from a Gram-positive bacterium. Wzb and CpsB have completely different structures, suggesting that they must operate by very different mechanisms. Although the mechanism of Wzb can be inferred from previous studies, CpsB appears to have a tyrosine phosphatase mechanism not observed before. We propose a chemical mechanism for CpsB based on site-directed mutagenesis and structural data. (C) 2009 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)678-688
Number of pages11
JournalJournal of Molecular Biology
Volume392
Issue number3
DOIs
Publication statusPublished - 25 Sept 2009

Keywords

  • enzyme mechanism
  • kinase
  • phosphatase
  • pathogenic bacteria
  • capsule
  • PHOSPHOTYROSINE-PROTEIN PHOSPHATASE
  • SITE-DIRECTED MUTAGENESIS
  • BACILLUS-SUBTILIS
  • CRYSTALLOGRAPHIC STRUCTURE
  • MEMBRANE-PROTEIN
  • GROUP-1 CAPSULES
  • PHOSPHORYLATION
  • POLYSACCHARIDES
  • BIOSYNTHESIS
  • SUBSTRATE

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