Critical role of LuxS in the virulence of Campylobacter jejuni in a guinea pig model of abortion

Paul Plummer, Orhan Sahin, Eric Burrough, Rachel Sippy, Kathy Mou, Jessica Rabenold, Mike Yaeger, Qijing Zhang

Research output: Contribution to journalArticlepeer-review


Previous studies on Campylobacter jejuni have demonstrated the role of LuxS in motility, cytolethal distending toxin production, agglutination, and intestinal colonization; however, its direct involvement in virulence has not been reported. In this study, we demonstrate a direct role of luxS in the virulence of C. jejuni in two different animal hosts. The IA3902 strain, a highly virulent sheep abortion strain recently described by our laboratory, along with its isogenic luxS mutant and luxS complement strains, was inoculated by the oral route into both a pregnant guinea pig virulence model and a chicken colonization model. In both cases, the IA3902 luxS mutant demonstrated a complete loss of ability to colonize the intestinal tract. In the pregnant model, the mutant also failed to induce abortion, while the wild-type strain was highly abortifacient. Genetic complementation of the luxS gene fully restored the virulent phenotype in both models. Interestingly, when the organism was inoculated into guinea pigs by the intraperitoneal route, no difference in virulence (abortion induction) was observed between the luxS mutant and the wild-type strain, suggesting that the defect in virulence following oral inoculation is likely associated with a defect in colonization and/or translocation of the organism out of the intestine. These studies provide the first direct evidence that LuxS plays an important role in the virulence of C. jejuni using an in vivo model of natural disease.

Original languageEnglish
Pages (from-to)585-93
Number of pages9
JournalInfection and Immunity
Issue number2
Publication statusPublished - Feb 2012


  • Abortion, Septic/microbiology
  • Animals
  • Bacterial Proteins/genetics
  • Campylobacter Infections/microbiology
  • Campylobacter jejuni/metabolism
  • Carbon-Sulfur Lyases/genetics
  • Chickens
  • Disease Models, Animal
  • Female
  • Guinea Pigs
  • Intestines/microbiology
  • Mutation
  • Pregnancy
  • Pregnancy Complications, Infectious/microbiology
  • Virulence


Dive into the research topics of 'Critical role of LuxS in the virulence of Campylobacter jejuni in a guinea pig model of abortion'. Together they form a unique fingerprint.

Cite this