Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis

C Berry; S O'Neil; E Ben-Dov; AF Jones; L Murphy; MA Quail; MTG Holden; D Harris; A Zaritsky; J Parkhill

doi:10.1128/AEM.68.10.5082-5092.2002

Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis

C Berry^*, S O'Neil, E Ben-Dov, AF Jones, L Murphy, MA Quail, MTG Holden, D Harris, A Zaritsky, J Parkhill

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

267 Citations (Scopus)

Abstract

The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.

Original language	English
Pages (from-to)	5082-5095
Number of pages	14
Journal	Applied and Environmental Microbiology
Volume	68
Issue number	10
DOIs	https://doi.org/10.1128/AEM.68.10.5082-5092.2002
Publication status	Published - Oct 2002

Keywords

PEPTIDE ANTIBIOTIC AS-48
NUCLEOTIDE-SEQUENCE
SPORE GERMINATION
TRANSCRIPTIONAL REGULATION
ENTEROCOCCUS-FAECALIS
CRYSTAL PROTEINS
ESCHERICHIA-COLI
RESTRICTION MAP
MOSQUITO LARVAE
RNA-POLYMERASE

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10.1128/AEM.68.10.5082-5092.2002

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title = "Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis",

abstract = "The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.",

keywords = "PEPTIDE ANTIBIOTIC AS-48, NUCLEOTIDE-SEQUENCE, SPORE GERMINATION, TRANSCRIPTIONAL REGULATION, ENTEROCOCCUS-FAECALIS, CRYSTAL PROTEINS, ESCHERICHIA-COLI, RESTRICTION MAP, MOSQUITO LARVAE, RNA-POLYMERASE",

author = "C Berry and S O'Neil and E Ben-Dov and AF Jones and L Murphy and MA Quail and MTG Holden and D Harris and A Zaritsky and J Parkhill",

year = "2002",

month = oct,

doi = "10.1128/AEM.68.10.5082-5092.2002",

language = "English",

volume = "68",

pages = "5082--5095",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "10",

}

TY - JOUR

T1 - Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis

AU - Berry, C

AU - O'Neil, S

AU - Ben-Dov, E

AU - Jones, AF

AU - Murphy, L

AU - Quail, MA

AU - Holden, MTG

AU - Harris, D

AU - Zaritsky, A

AU - Parkhill, J

PY - 2002/10

Y1 - 2002/10

N2 - The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.

AB - The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.

KW - PEPTIDE ANTIBIOTIC AS-48

KW - NUCLEOTIDE-SEQUENCE

KW - SPORE GERMINATION

KW - TRANSCRIPTIONAL REGULATION

KW - ENTEROCOCCUS-FAECALIS

KW - CRYSTAL PROTEINS

KW - ESCHERICHIA-COLI

KW - RESTRICTION MAP

KW - MOSQUITO LARVAE

KW - RNA-POLYMERASE

U2 - 10.1128/AEM.68.10.5082-5092.2002

DO - 10.1128/AEM.68.10.5082-5092.2002

M3 - Article

SN - 0099-2240

VL - 68

SP - 5082

EP - 5095

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis

Abstract

Keywords

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