Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18

J Parkhill; G Dougan; KD James; NR Thomson; D Pickard; J Wain; C Churcher; KL Mungall; SD Bentley; MTG Holden; M Sebaihia; S Baker; D Basham; K Brooks; T Chillingworth; P Connerton; A Cronin; P Davis; RM Davies; L Dowd; N White; J Farrar; T Feltwell; N Hamlin; A Haque; TT Hien; S Holroyd; K Jagels; A Krogh; TS Larsen; S Leather; S Moule; P O'Gaora; C Parry; M Quail; K Rutherford; M Simmonds; J Skelton; K Stevens; S Whitehead; BG Barrell

Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18

J Parkhill^*, G Dougan, KD James, NR Thomson, D Pickard, J Wain, C Churcher, KL Mungall, SD Bentley, MTG Holden, M Sebaihia, S Baker, D Basham, K Brooks, T Chillingworth, P Connerton, A Cronin, P Davis, RM Davies, L DowdN White, J Farrar, T Feltwell, N Hamlin, A Haque, TT Hien, S Holroyd, K Jagels, A Krogh, TS Larsen, S Leather, S Moule, P O'Gaora, C Parry, M Quail, K Rutherford, M Simmonds, J Skelton, K Stevens, S Whitehead, BG Barrell

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Salmonella enterica serovar Typhi (S. typhi) is the aetiological agent of typhoid fever, a serious invasive bacterial disease of humans with an annual global burden of approximately 16 million cases, leading to 600,000 fatalities(1). Many S. enterica serovars actively invade the mucosal surface of the intestine but are normally contained in healthy individuals by the local immune defence mechanisms. However, S. typhi has evolved the ability to spread to the deeper tissues of humans, including liver, spleen and bone marrow. Here we have sequenced the 4,809,037-base pair (bp) genome of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands. Notably, the genome sequence identifies over two hundred pseudogenes, several corresponding to genes that are known to contribute to virulence in Salmonella typhimurium. This genetic degradation may contribute to the human-restricted host range for S. typhi. CT18 harbours a 218,150-bp multiple-drug-resistance incH1 plasmid (pHCM1), and a 106,516-bp cryptic plasmid (pHCM2), which shows recent common ancestry with a virulence plasmid of Yersinia pestis.

Original language	English
Pages (from-to)	848-852
Number of pages	5
Journal	Nature
Volume	413
Issue number	6858
Publication status	Published - 25 Oct 2001

Keywords

COMPLETE DNA-SEQUENCE
PATHOGENICITY ISLAND
EPITHELIAL-CELLS
YERSINIA-PESTIS
TYPHIMURIUM
IDENTIFICATION
PROTEIN
VIRULENCE
BACTERIA
STRAIN

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Parkhill, J., Dougan, G., James, KD., Thomson, NR., Pickard, D., Wain, J., Churcher, C., Mungall, KL., Bentley, SD., Holden, MTG., Sebaihia, M., Baker, S., Basham, D., Brooks, K., Chillingworth, T., Connerton, P., Cronin, A., Davis, P., Davies, RM., ... Barrell, BG. (2001). Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature, 413(6858), 848-852.

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title = "Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18",

abstract = "Salmonella enterica serovar Typhi (S. typhi) is the aetiological agent of typhoid fever, a serious invasive bacterial disease of humans with an annual global burden of approximately 16 million cases, leading to 600,000 fatalities(1). Many S. enterica serovars actively invade the mucosal surface of the intestine but are normally contained in healthy individuals by the local immune defence mechanisms. However, S. typhi has evolved the ability to spread to the deeper tissues of humans, including liver, spleen and bone marrow. Here we have sequenced the 4,809,037-base pair (bp) genome of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands. Notably, the genome sequence identifies over two hundred pseudogenes, several corresponding to genes that are known to contribute to virulence in Salmonella typhimurium. This genetic degradation may contribute to the human-restricted host range for S. typhi. CT18 harbours a 218,150-bp multiple-drug-resistance incH1 plasmid (pHCM1), and a 106,516-bp cryptic plasmid (pHCM2), which shows recent common ancestry with a virulence plasmid of Yersinia pestis.",

keywords = "COMPLETE DNA-SEQUENCE, PATHOGENICITY ISLAND, EPITHELIAL-CELLS, YERSINIA-PESTIS, TYPHIMURIUM, IDENTIFICATION, PROTEIN, VIRULENCE, BACTERIA, STRAIN",

author = "J Parkhill and G Dougan and KD James and NR Thomson and D Pickard and J Wain and C Churcher and KL Mungall and SD Bentley and MTG Holden and M Sebaihia and S Baker and D Basham and K Brooks and T Chillingworth and P Connerton and A Cronin and P Davis and RM Davies and L Dowd and N White and J Farrar and T Feltwell and N Hamlin and A Haque and TT Hien and S Holroyd and K Jagels and A Krogh and TS Larsen and S Leather and S Moule and P O'Gaora and C Parry and M Quail and K Rutherford and M Simmonds and J Skelton and K Stevens and S Whitehead and BG Barrell",

year = "2001",

month = oct,

day = "25",

language = "English",

volume = "413",

pages = "848--852",

journal = "Nature",

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Parkhill, J, Dougan, G, James, KD, Thomson, NR, Pickard, D, Wain, J, Churcher, C, Mungall, KL, Bentley, SD, Holden, MTG, Sebaihia, M, Baker, S, Basham, D, Brooks, K, Chillingworth, T, Connerton, P, Cronin, A, Davis, P, Davies, RM, Dowd, L, White, N, Farrar, J, Feltwell, T, Hamlin, N, Haque, A, Hien, TT, Holroyd, S, Jagels, K, Krogh, A, Larsen, TS, Leather, S, Moule, S, O'Gaora, P, Parry, C, Quail, M, Rutherford, K, Simmonds, M, Skelton, J, Stevens, K, Whitehead, S & Barrell, BG 2001, 'Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18', Nature, vol. 413, no. 6858, pp. 848-852.

TY - JOUR

T1 - Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18

AU - Parkhill, J

AU - Dougan, G

AU - James, KD

AU - Thomson, NR

AU - Pickard, D

AU - Wain, J

AU - Churcher, C

AU - Mungall, KL

AU - Bentley, SD

AU - Holden, MTG

AU - Sebaihia, M

AU - Baker, S

AU - Basham, D

AU - Brooks, K

AU - Chillingworth, T

AU - Connerton, P

AU - Cronin, A

AU - Davis, P

AU - Davies, RM

AU - Dowd, L

AU - White, N

AU - Farrar, J

AU - Feltwell, T

AU - Hamlin, N

AU - Haque, A

AU - Hien, TT

AU - Holroyd, S

AU - Jagels, K

AU - Krogh, A

AU - Larsen, TS

AU - Leather, S

AU - Moule, S

AU - O'Gaora, P

AU - Parry, C

AU - Quail, M

AU - Rutherford, K

AU - Simmonds, M

AU - Skelton, J

AU - Stevens, K

AU - Whitehead, S

AU - Barrell, BG

PY - 2001/10/25

Y1 - 2001/10/25

N2 - Salmonella enterica serovar Typhi (S. typhi) is the aetiological agent of typhoid fever, a serious invasive bacterial disease of humans with an annual global burden of approximately 16 million cases, leading to 600,000 fatalities(1). Many S. enterica serovars actively invade the mucosal surface of the intestine but are normally contained in healthy individuals by the local immune defence mechanisms. However, S. typhi has evolved the ability to spread to the deeper tissues of humans, including liver, spleen and bone marrow. Here we have sequenced the 4,809,037-base pair (bp) genome of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands. Notably, the genome sequence identifies over two hundred pseudogenes, several corresponding to genes that are known to contribute to virulence in Salmonella typhimurium. This genetic degradation may contribute to the human-restricted host range for S. typhi. CT18 harbours a 218,150-bp multiple-drug-resistance incH1 plasmid (pHCM1), and a 106,516-bp cryptic plasmid (pHCM2), which shows recent common ancestry with a virulence plasmid of Yersinia pestis.

AB - Salmonella enterica serovar Typhi (S. typhi) is the aetiological agent of typhoid fever, a serious invasive bacterial disease of humans with an annual global burden of approximately 16 million cases, leading to 600,000 fatalities(1). Many S. enterica serovars actively invade the mucosal surface of the intestine but are normally contained in healthy individuals by the local immune defence mechanisms. However, S. typhi has evolved the ability to spread to the deeper tissues of humans, including liver, spleen and bone marrow. Here we have sequenced the 4,809,037-base pair (bp) genome of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands. Notably, the genome sequence identifies over two hundred pseudogenes, several corresponding to genes that are known to contribute to virulence in Salmonella typhimurium. This genetic degradation may contribute to the human-restricted host range for S. typhi. CT18 harbours a 218,150-bp multiple-drug-resistance incH1 plasmid (pHCM1), and a 106,516-bp cryptic plasmid (pHCM2), which shows recent common ancestry with a virulence plasmid of Yersinia pestis.

KW - COMPLETE DNA-SEQUENCE

KW - PATHOGENICITY ISLAND

KW - EPITHELIAL-CELLS

KW - YERSINIA-PESTIS

KW - TYPHIMURIUM

KW - IDENTIFICATION

KW - PROTEIN

KW - VIRULENCE

KW - BACTERIA

KW - STRAIN

M3 - Article

SN - 0028-0836

VL - 413

SP - 848

EP - 852

JO - Nature

JF - Nature

IS - 6858

ER -

Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18

Abstract

Keywords

UN SDGs

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