Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

Janine T. Bossé; Yanwen Li; Liza Miriam Cohen; Marc Stegger; Øystein Angen; Sonia Lacouture; Marcelo Gottschalk; Liancheng Lei; Miriam Koene; Peter Kuhnert; Aloka B. Bandara; Thomas J. Inzana; Matthew T. G. Holden; David Harris; Olusegun Oshota; Duncan J. Maskell; Alexander W. Tucker; Brendan W. Wren; Andrew N. Rycroft; Paul R. Langford; BRaDP1T Consortium

doi:10.1099/mgen.0.000687

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

Janine T. Bossé, Yanwen Li, Liza Miriam Cohen, Marc Stegger, Øystein Angen, Sonia Lacouture, Marcelo Gottschalk, Liancheng Lei, Miriam Koene, Peter Kuhnert, Aloka B. Bandara, Thomas J. Inzana, Matthew T. G. Holden, David Harris, Olusegun Oshota, Duncan J. Maskell, Alexander W. Tucker, Brendan W. Wren, Andrew N. Rycroft, Paul R. LangfordBRaDP1T Consortium

Research output: Contribution to journal › Article › peer-review

Abstract

We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

Original language	English
Article number	687
Number of pages	7
Journal	Microbial Genomics
Volume	7
Issue number	11
DOIs	https://doi.org/10.1099/mgen.0.000687
Publication status	Published - 24 Nov 2021

Keywords

Serovar 8 reference genome
SNPs
Actinobacillus pleuropneumoniae

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Bosse_2021_MG_Complete-genome-A.pleuropneumoniae_CC
Copyright © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Bossé, J. T., Li, Y., Cohen, L. M., Stegger, M., Angen, Ø., Lacouture, S., Gottschalk, M., Lei, L., Koene, M., Kuhnert, P., Bandara, A. B., Inzana, T. J., Holden, M. T. G., Harris, D., Oshota, O., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., ... BRaDP1T Consortium (2021). Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation. Microbial Genomics, 7(11), Article 687. https://doi.org/10.1099/mgen.0.000687

@article{7ab8b890360c49c2b496262ac3271f7c,

title = "Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation",

abstract = "We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.",

keywords = "Serovar 8 reference genome, SNPs, Actinobacillus pleuropneumoniae",

author = "Boss{\'e}, {Janine T.} and Yanwen Li and Cohen, {Liza Miriam} and Marc Stegger and {\O}ystein Angen and Sonia Lacouture and Marcelo Gottschalk and Liancheng Lei and Miriam Koene and Peter Kuhnert and Bandara, {Aloka B.} and Inzana, {Thomas J.} and Holden, {Matthew T. G.} and David Harris and Olusegun Oshota and Maskell, {Duncan J.} and Tucker, {Alexander W.} and Wren, {Brendan W.} and Rycroft, {Andrew N.} and Langford, {Paul R.} and {BRaDP1T Consortium}",

note = "This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, BB/G018553/1, BB/S002103/1, and BB/S005897/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. Funding for LL provided by the {\textquoteleft}National Natural Science Foundation of China{\textquoteright} (No.31520103917). MTGH and DH were supported by the Wellcome Trust (grant number 098051). ",

year = "2021",

month = nov,

day = "24",

doi = "10.1099/mgen.0.000687",

language = "English",

volume = "7",

journal = "Microbial Genomics",

issn = "2057-5858",

publisher = "Microbiology Society",

number = "11",

}

Bossé, JT, Li, Y, Cohen, LM, Stegger, M, Angen, Ø, Lacouture, S, Gottschalk, M, Lei, L, Koene, M, Kuhnert, P, Bandara, AB, Inzana, TJ, Holden, MTG, Harris, D, Oshota, O, Maskell, DJ, Tucker, AW, Wren, BW, Rycroft, AN, Langford, PR & BRaDP1T Consortium 2021, 'Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation', Microbial Genomics, vol. 7, no. 11, 687. https://doi.org/10.1099/mgen.0.000687

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation. / Bossé, Janine T.; Li, Yanwen; Cohen, Liza Miriam et al.
In: Microbial Genomics, Vol. 7, No. 11, 687, 24.11.2021.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405

T2 - comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

AU - Bossé, Janine T.

AU - Li, Yanwen

AU - Cohen, Liza Miriam

AU - Stegger, Marc

AU - Angen, Øystein

AU - Lacouture, Sonia

AU - Gottschalk, Marcelo

AU - Lei, Liancheng

AU - Koene, Miriam

AU - Kuhnert, Peter

AU - Bandara, Aloka B.

AU - Inzana, Thomas J.

AU - Holden, Matthew T. G.

AU - Harris, David

AU - Oshota, Olusegun

AU - Maskell, Duncan J.

AU - Tucker, Alexander W.

AU - Wren, Brendan W.

AU - Rycroft, Andrew N.

AU - Langford, Paul R.

AU - BRaDP1T Consortium

N1 - This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, BB/G018553/1, BB/S002103/1, and BB/S005897/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. Funding for LL provided by the ‘National Natural Science Foundation of China’ (No.31520103917). MTGH and DH were supported by the Wellcome Trust (grant number 098051).

PY - 2021/11/24

Y1 - 2021/11/24

N2 - We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

AB - We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

KW - Serovar 8 reference genome

KW - SNPs

KW - Actinobacillus pleuropneumoniae

U2 - 10.1099/mgen.0.000687

DO - 10.1099/mgen.0.000687

M3 - Article

SN - 2057-5858

VL - 7

JO - Microbial Genomics

JF - Microbial Genomics

IS - 11

M1 - 687

ER -

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

Abstract

Keywords

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