Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

Janine T Bossé, Yanwen Li, Roberto Fernandez Crespo, Sonia Lacouture, Marcelo Gottschalk, Rita Sárközi, László Fodor, Maria Casas Amoribieta, Øystein Angen, Katerina Nedbalcova, Matthew T. G. Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford, BRaDP1T Consortium

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalVeterinary Microbiology
Volume220
Early online date20 May 2018
DOIs
Publication statusPublished - Jul 2018

Keywords

  • Diagnostic
  • mPCR
  • Capsule typing
  • A. pleuropneumoniae
  • Serovars

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