Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus

Angelika Ziegler, Graham Cowan, Lesley Torrance

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis revealed a close relationship with Panicum mosaic virus (PMV; genus Panicovirus), and together with serological studies, the work supports the classification of CMMV in the family Tombusviridae, genus Panicovirus rather than, as is currently proposed, the genus Sobemovirus. A full-length cDNA clone was prepared, and RNA transcripts synthesised from cDNA were shown to be infectious when inoculated to Hordeum vulgare.

Original languageEnglish
Pages (from-to)1545-1549
Number of pages5
JournalArchives of Virology
Volume154
Issue number9
DOIs
Publication statusPublished - Sept 2009

Keywords

  • NUCLEOTIDE-SEQUENCE
  • PLANT-VIRUSES
  • RNA VIRUSES
  • GENOMIC RNA
  • TRANSLATION
  • PROTEINS
  • INITIATION
  • PHYLOGENY

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