Comparative effect of ALA derivatives on protoporphyrin IX production in human and rat skin organ cultures

A Casas, A M D Batlle, A R Butler, D Robertson, E H Brown, A MacRobert, P A Riley

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80 Citations (Scopus)

Abstract

Samples of human and rat skin in short-term organ culture exposed to ALA or a range of hydrophobic derivatives were examined for their effect on the accumulation of protoporphyrin IX (PpIX) measured using fluorescence spectroscopy. With the exception of carbobenzoyloxy-D-phenylalanyl-5-ALA-ethyl ester the data presented indicate that, in normal tissues, ALA derivatives generate protoporphyrin IX more slowly than ALA, suggesting that they are less rapidly taken up and/or converted to free ALA. However, the resultant depot effect may lead to the enhanced accumulation of porphyrin over long exposure periods, particularly in the case of ALA-methyl ester or ALA-hexyl ester, depending on the applied concentration and the exposed tissue. Addition of the iron chelator, CP94, greatly increased PpIX accumulation in human skin exposed to ALA, ALA-methyl ester and ALA-hexyl ester. The effect in rat skin was less marked.

Original languageEnglish
Pages (from-to)1525-1532
Number of pages8
JournalBritish Journal of Cancer
Volume80
Publication statusPublished - Jul 1999

Keywords

  • ALA
  • PDT
  • ALA derivatives
  • ALA esters
  • iron chelators
  • CP94
  • DELTA-AMINOLEVULINIC-ACID
  • 5-AMINOLEVULINIC ACID
  • PHOTODYNAMIC THERAPY
  • INDUCED PORPHYRIN
  • TREATED CELLS
  • ACCUMULATION
  • PHOTODESTRUCTION
  • FLUORESCENCE
  • ENHANCEMENT
  • TRANSPORT

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