Combined imaging and chemical sensing of L-glutamate release from the foregut plexus of the Lepidopteran, Manduca sexta

J. P. Issberner*, C. L. Schauer, B. A. Trimmer, D. R. Walt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A new combined imaging and chemical detection sensor for the measurement of localized L-glutamate release at the insect neuromuscular junction (NMJ) is presented. The sensor is comprised of an L-glutamate-sensitive fluorescent gel, spin-coated onto the tip of an optical imaging fiber. The gel is composed of L-glutamate oxidase (GLOD); a pH-sensitive fluorescent dye, SNAFL; and poly(acrylamide-co-N-acryloxysuccinimide) (PAN). NH3 is liberated from the interaction of L-glutamate with GLOD, which reversibly reduces the emitted fluorescence signal from SNAFL. This sensor has a spatial resolution of 3-4 μm, and an L-glutamate detection limit of between 10 and 100 μM. L-glutamate release and re-uptake from the foregut plexus of Manduca sexta was detected by the sensor in the presence of the L-glutamate re-uptake blocker dihydrokainate, and the post-synaptic L-glutamate receptor antagonist CNQX.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalJournal of Neuroscience Methods
Volume120
Issue number1
DOIs
Publication statusPublished - 15 Oct 2002

Keywords

  • Detection
  • Fluorescence-based biosensor
  • Insect
  • L-glutamate
  • Manduca
  • Neurotransmitter

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