Co-incident signalling between the Gi/Go-coupled d-opoid receptor and the Gq-coupled m3 muscarinic receptor at the level of intracellular free calcium in SH-SY5Y cells

A Yeo, DSK Samways, CE Fowler, Francis James Gunn-Moore, G Henderson

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

In SH-SY5Y cells, activation of delta -opioid receptors with [D-Pen(2,5)]-enkephalin (DPDPE; 1 muM) did not alter the intracellular free Ca2+ concentration [Ca2+](i). However, when DPDPE was applied during concomitant Gq-coupled m3 muscarinic receptor stimulation by carbachol or oxotremorine-M, it produced an elevation of [Ca2+](i). The DPDPE-evoked increase in [Ca2+](i) was abolished when the carbachol-sensitive intracellular Ca2+ store was emptied. There was a marked difference between the concentration-response relationship for the elevation of [Ca2+](i) by carbachol (EC50 13 muM, Hill slope 1) and the concentration-response relationship for carbachol's permissive action in revealing the delta -opioid receptor-mediated elevation of [Ca2+] (EC50 0.7 mM; Hill slope 1.8). Sequestration of free G protein beta gamma dimers by transient transfection of cells with a beta gamma binding protein (residues 495-689 of the C terminal tail of G protein-coupled receptor kinase 2) reduced the ability of delta opioid receptor activation to elevate [Ca2+](i). However, DPDPE did not elevate either basal or oxotremorine-M-evoked inositol phosphate production indicating that delta -opioid receptor activation did not stimulate phospholipase C. Furthermore, delta -opioid receptor activation did not result in the reversal of muscarinic receptor desensitization, membrane hyperpolarization or stimulation of sphingosine kinase. There was no coincident signalling between the delta -opioid receptor and the lysophosphatidic acid receptor which couples to elevation of [Ca2+](i) in SH-SY5Y cells by a PLC-independent mechanism. In SH-SY5Y cells the coincident signalling between the endogenously expressed delta -opioid and m3 muscarinic receptors appears to occur in the receptor activation-Ca2+ release signalling pathway at a step after the activation of phospholipase C.

Original languageEnglish
Pages (from-to)1688-1700
Number of pages13
JournalJournal of Neurochemistry
Volume76
Publication statusPublished - Mar 2001

Keywords

  • Ca2+ mobilization
  • delta-opioid receptors
  • m3 muscarinic receptors
  • phospholipase C
  • receptor cross-talk
  • SH-SY5Y cells
  • BETA-GAMMA-SUBUNITS
  • HUMAN NEUROBLASTOMA-CELLS
  • HETEROTRIMERIC G-PROTEINS
  • PHOSPHOLIPASE-C ISOZYMES
  • SENSITIVE G-PROTEINS
  • NG 108-15 CELLS
  • SPHINGOSINE KINASE
  • CROSS-TALK
  • INOSITOL 1,4,5-TRISPHOSPHATE
  • G(I)-COUPLED RECEPTORS

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