Cloning, expression and functional characterisation of a peroxiredoxin from the potato cyst nematode Globedera rostochiensis

L Robertson*, WM Robertson, M Sobczak, J Helder, E Tetaud, MR Ariyanayagam, MAJ Ferguson, A Fairlamb, JT Jones

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)

Abstract

We report the cloning, expression and functional characterisation of a peroxidase belonging to the peroxiredoxin family from the potato cyst nematode Globodera rostochiensis, the first molecule of this type from any nematode parasitic on plants. The G. rostochiensis peroxiredoxin catalyses the breakdown of hydrogen peroxide, but not cumene or t-butyl hydroperoxide, in a trypanosomatid reducing system comprising trypanothione reductase, trypanothione and tryparedoxin. In common with its homologues from Onchocerca volvulus and Brugia malayi, the G. rostochiensis enzyme is present on the surface of invasive and post-infective juveniles despite the apparent lack of a cleavable N-terminal signal peptide. The possibility that the G. rostochiensis peroxiredoxin plays a role in protection of the parasite from plant defence responses is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume111
Issue number1
Publication statusPublished - Nov 2000

Keywords

  • Globodera rostochiensis
  • peroxiredoxin
  • secretions
  • antioxidant protein
  • CRITHIDIA-FASCICULATA
  • THIOREDOXIN PEROXIDASE
  • ONCHOCERCA-VOLVULUS
  • MOLECULAR-CLONING
  • PLANT
  • GLUTATHIONE
  • METABOLISM
  • SECRETIONS
  • PARASITES

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