Cloning and sequencing of the nifH gene of Desulfovibrio gigas

H M Kent, M Buck, D J Evans

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The Desulfovibrio gigas nifH gene has been cloned and sequenced. It consists of an open-reading frame of 822 base pairs encoding a 274 amino acid polypeptide. A potential ntrA-dependent promotor sequence is present. The gene lacks an upstream activator sequence homologous to those often found in nif genes subject to activation by nifA.
Original languageEnglish
Pages (from-to)73-8
Number of pages6
JournalFEMS Microbiology Letters
Volume52
Issue number1-2
Publication statusPublished - 1 Oct 1989

Keywords

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Desulfovibrio
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Molecular Sequence Data
  • Nitrogen Fixation
  • Restriction Mapping

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