Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes

S. R.  Clingenpeel; S. D'Imperio; Harry Daniel Oduro; G. K. Druschel; T. R. McDermott

doi:10.1128/AEM.00336-09

Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes

S. R. Clingenpeel, S. D'Imperio, Harry Daniel Oduro, G. K. Druschel, T. R. McDermott

Research output: Contribution to journal › Article › peer-review

Abstract

Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.

Original language	English
Pages (from-to)	3362-3365
Journal	Applied and Environmental Microbiology
Volume	75
DOIs	https://doi.org/10.1128/AEM.00336-09
Publication status	Published - Mar 2009

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title = "Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes",

abstract = "Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat. ",

author = "Clingenpeel, {S. R.} and S. D'Imperio and Oduro, {Harry Daniel} and Druschel, {G. K.} and McDermott, {T. R.}",

year = "2009",

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T1 - Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes

AU - Clingenpeel, S. R.

AU - D'Imperio, S.

AU - Oduro, Harry Daniel

AU - Druschel, G. K.

AU - McDermott, T. R.

PY - 2009/3

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AB - Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.

UR - http://aem.asm.org/content/75/10/3362.abstract?sid=91f0a31f-3777-4d47-bd88-13259cc00bd8

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DO - 10.1128/AEM.00336-09

M3 - Article

SN - 0099-2240

VL - 75

SP - 3362

EP - 3365

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

ER -

Cloning and In Situ Expression Studies of the Hydrogenobaculum Arsenite Oxidase Genes

Abstract

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