Cloning and Expression of two isoforms of guanylate cyclase C (GC-C) in the European Eel (Anguilla anguilla).

M Comrie, Christopher Paul Cutler, Gordon Cramb

Research output: Other contribution

20 Citations (Scopus)

Abstract

Complementary DNA fragments for two isoforms of particulate guanylate cyclase C (GC-C) were cloned from the intestine of the European eel (Anguilla anguilla). Both isoforms exhibited higher nucleotide and amino acid sequence homologies to members of the GC-C family from other species than the related guanylate cyclase A or B (GC-A or GC-B) isoforms from the eel. Northern blots indicated that probes for both isoforms, termed GC-C1 and GC-C2, selectively hybridised to 4.8-kb transcripts in the intestine and the kidney. Expression of the GC-C2 transcript in the intestine was increased by 100% following the transfer of yellow FW-acclimated eels to SW. Likewise developmental maturation of yellow eels into pre-migratory silver eels resulted in a significant increase (60%) in the intestinal expression of GC-C2. No changes in expression of GC-C2 were seen in the kidney under any condition. RT-PCR indicated that the GC-C2 isoform is only expressed in anterior and mid-gut segments in FW-acclimated yellow eels. However, expression is also extended to the posterior gut segment when yellow eels are acclimated to SW or following developmental transformation into silver eels. (C) 2001 Elsevier Science Inc. All rights reserved.

Original languageEnglish
Volume129
Publication statusPublished - Jun 2001

Keywords

  • Anguilla
  • eel
  • intestine
  • nucleotide sequence
  • guanylate cyclase
  • GC-C
  • RT-PCR
  • NATRIURETIC-PEPTIDE RECEPTOR
  • HEAT-STABLE ENTEROTOXIN
  • TISSUE DISTRIBUTION
  • INTESTINAL-TRACT
  • OPOSSUM KIDNEY
  • CYCLIC-GMP
  • UROGUANYLIN
  • INHIBITION
  • TRANSPORT
  • SECRETION

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