Clonal chromosomal abberations in simian virus 40-transfected human thyroid cells and in derived tumours developed after in vitro irradiation.

H Zitzelsberger, J Bruch, J Smida, L Hieber, Clare Peddie, Peter Edward Bryant, Andrew Clive Riches, F Jingly, G Heinz-Ulrich, M Bauchinger

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In vitro model cell systems are important tools for studying mechanisms of radiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma -irradiation and subsequent tumor formation in at athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as cs ell as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation-induced malignant transformation in human thyroid cells. (C) 2001 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)166-177
Number of pages12
JournalInternational Journal of Cancer
Volume96
Publication statusPublished - 20 Jun 2001

Keywords

  • in vitro model cell system
  • radiation-induced tumorigenesis
  • chromosomal aberration
  • comparative genomic hybridization
  • spectral karyotyping
  • COMPARATIVE GENOMIC HYBRIDIZATION
  • RADIATION-INDUCED TRANSFORMATION
  • COPY NUMBER CHANGES
  • EPITHELIAL-CELLS
  • CELLULAR SENESCENCE
  • NEOPLASTIC TRANSFORMATION
  • INDEFINITE DIVISION
  • IONIZING-RADIATION
  • ALPHA-PARTICLES
  • SOLID TUMORS

Fingerprint

Dive into the research topics of 'Clonal chromosomal abberations in simian virus 40-transfected human thyroid cells and in derived tumours developed after in vitro irradiation.'. Together they form a unique fingerprint.

Cite this