TY - JOUR
T1 - Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo
AU - Stramer, Brian
AU - Moreira, Severina
AU - Millard, Tom
AU - Evans, Iwan
AU - Huang, Chieh-Yin
AU - Sabet, Ola
AU - Milner, Martin John
AU - Dunn, Graham
AU - Martin, Paul
AU - Wood, Will
N1 - P. Martin and W. Wood contributed equally to this paper
PY - 2010/5/17
Y1 - 2010/5/17
N2 - Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
AB - Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
UR - http://www.scopus.com/inward/record.url?scp=77952335884&partnerID=8YFLogxK
UR - http://jcb.rupress.org/content/189/4/681.abstract
U2 - 10.1083/jcb.200912134
DO - 10.1083/jcb.200912134
M3 - Article
SN - 0021-9525
VL - 189
SP - 681
EP - 689
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 4
ER -