Chitosan-GSNO nanoparticles: a positive modulator of drought stress tolerance in soybean

Nusrat Jahan Methela; Anjali Pande; Mohammad Shafiqul Islam; Waqas Rahim; Adil Hussain; Da-Sol Lee; Bong-Gyu Mun; Nirmal Prashanth Maria Joseph Raj; Sang-Jae Kim; Yoonha Kim; Byung-Wook Yun

doi:10.1186/s12870-023-04640-x

Chitosan-GSNO nanoparticles: a positive modulator of drought stress tolerance in soybean

Nusrat Jahan Methela, Anjali Pande, Mohammad Shafiqul Islam, Waqas Rahim, Adil Hussain^*, Da-Sol Lee, Bong-Gyu Mun, Nirmal Prashanth Maria Joseph Raj, Sang-Jae Kim, Yoonha Kim, Byung-Wook Yun^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Chitosan biopolymer is an emerging non-toxic and biodegradable plant elicitor or bio-stimulant. Chitosan nanoparticles (CSNPs) have been used for the enhancement of plant growth and development. On the other hand, NO is an important signaling molecule that regulates several aspects of plant physiology under normal and stress conditions. Here we report the synthesis, characterization, and use of chitosan-GSNO nanoparticles for improving drought stress tolerance in soybean.
Results: The CSGSNONPs released NO gas for a significantly longer period and at a much lower rate as compared to free GSNO indicating that incorporation of GSNO in CSNPs can protect the NO-donor from rapid decomposition and ensure optimal NO release. CS-GSNONPs improved drought tolerance in soybean plants reflected by a significant increase in plant height, biomass, root length, root volume, root surface area, number of root tips, forks, and nodules. Further analyses indicated significantly lower electrolyte leakage, higher proline content, higher catalase, and ascorbate peroxidase activity, and reduction in MDA and H2O2 contents after treatment with 50 μM CS-GSNONPs under drought stress conditions. Quantitative real-time PCR analysis indicated that CS-GSNONPs protected against drought-induced stress by regulating the expression of drought stress-related marker genes such as GmDREB1a, GmP5CS, GmDEFENSIN, and NO-related genes GmGSNOR1 and GmNOX1.
Conclusions: This study highlights the potential of nano-technology-based delivery systems for nitric oxide donors to improve plant growth, and development and protect against stresses.

Original language	English
Article number	639
Number of pages	15
Journal	BMC Plant Biology
Volume	23
Early online date	11 Dec 2023
DOIs	https://doi.org/10.1186/s12870-023-04640-x
Publication status	E-pub ahead of print - 11 Dec 2023

Keywords

Chitosan
Nitric oxide
GSNO
Glycine max
Nanoparticles

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Cite this

@article{b65683b616414091a8cbd669a876361b,

title = "Chitosan-GSNO nanoparticles: a positive modulator of drought stress tolerance in soybean",

abstract = "Background: Chitosan biopolymer is an emerging non-toxic and biodegradable plant elicitor or bio-stimulant. Chitosan nanoparticles (CSNPs) have been used for the enhancement of plant growth and development. On the other hand, NO is an important signaling molecule that regulates several aspects of plant physiology under normal and stress conditions. Here we report the synthesis, characterization, and use of chitosan-GSNO nanoparticles for improving drought stress tolerance in soybean. Results: The CSGSNONPs released NO gas for a significantly longer period and at a much lower rate as compared to free GSNO indicating that incorporation of GSNO in CSNPs can protect the NO-donor from rapid decomposition and ensure optimal NO release. CS-GSNONPs improved drought tolerance in soybean plants reflected by a significant increase in plant height, biomass, root length, root volume, root surface area, number of root tips, forks, and nodules. Further analyses indicated significantly lower electrolyte leakage, higher proline content, higher catalase, and ascorbate peroxidase activity, and reduction in MDA and H2O2 contents after treatment with 50 μM CS-GSNONPs under drought stress conditions. Quantitative real-time PCR analysis indicated that CS-GSNONPs protected against drought-induced stress by regulating the expression of drought stress-related marker genes such as GmDREB1a, GmP5CS, GmDEFENSIN, and NO-related genes GmGSNOR1 and GmNOX1. Conclusions: This study highlights the potential of nano-technology-based delivery systems for nitric oxide donors to improve plant growth, and development and protect against stresses.",

keywords = "Chitosan, Nitric oxide, GSNO, Glycine max, Nanoparticles",

author = "Methela, {Nusrat Jahan} and Anjali Pande and Islam, {Mohammad Shafiqul} and Waqas Rahim and Adil Hussain and Da-Sol Lee and Bong-Gyu Mun and {Maria Joseph Raj}, {Nirmal Prashanth} and Sang-Jae Kim and Yoonha Kim and Byung-Wook Yun",

note = "Funding: This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education (RS-2023-00245922) to Prof. Byung-Wook Yun and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01049154) to Dr. Bong-Gyu Mun.",

year = "2023",

month = dec,

day = "11",

doi = "10.1186/s12870-023-04640-x",

language = "English",

volume = "23",

journal = "BMC Plant Biology",

issn = "1471-2229",

publisher = "BioMed Central",

}

TY - JOUR

T1 - Chitosan-GSNO nanoparticles

T2 - a positive modulator of drought stress tolerance in soybean

AU - Methela, Nusrat Jahan

AU - Pande, Anjali

AU - Islam, Mohammad Shafiqul

AU - Rahim, Waqas

AU - Hussain, Adil

AU - Lee, Da-Sol

AU - Mun, Bong-Gyu

AU - Maria Joseph Raj, Nirmal Prashanth

AU - Kim, Sang-Jae

AU - Kim, Yoonha

AU - Yun, Byung-Wook

N1 - Funding: This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education (RS-2023-00245922) to Prof. Byung-Wook Yun and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01049154) to Dr. Bong-Gyu Mun.

PY - 2023/12/11

Y1 - 2023/12/11

N2 - Background: Chitosan biopolymer is an emerging non-toxic and biodegradable plant elicitor or bio-stimulant. Chitosan nanoparticles (CSNPs) have been used for the enhancement of plant growth and development. On the other hand, NO is an important signaling molecule that regulates several aspects of plant physiology under normal and stress conditions. Here we report the synthesis, characterization, and use of chitosan-GSNO nanoparticles for improving drought stress tolerance in soybean. Results: The CSGSNONPs released NO gas for a significantly longer period and at a much lower rate as compared to free GSNO indicating that incorporation of GSNO in CSNPs can protect the NO-donor from rapid decomposition and ensure optimal NO release. CS-GSNONPs improved drought tolerance in soybean plants reflected by a significant increase in plant height, biomass, root length, root volume, root surface area, number of root tips, forks, and nodules. Further analyses indicated significantly lower electrolyte leakage, higher proline content, higher catalase, and ascorbate peroxidase activity, and reduction in MDA and H2O2 contents after treatment with 50 μM CS-GSNONPs under drought stress conditions. Quantitative real-time PCR analysis indicated that CS-GSNONPs protected against drought-induced stress by regulating the expression of drought stress-related marker genes such as GmDREB1a, GmP5CS, GmDEFENSIN, and NO-related genes GmGSNOR1 and GmNOX1. Conclusions: This study highlights the potential of nano-technology-based delivery systems for nitric oxide donors to improve plant growth, and development and protect against stresses.

AB - Background: Chitosan biopolymer is an emerging non-toxic and biodegradable plant elicitor or bio-stimulant. Chitosan nanoparticles (CSNPs) have been used for the enhancement of plant growth and development. On the other hand, NO is an important signaling molecule that regulates several aspects of plant physiology under normal and stress conditions. Here we report the synthesis, characterization, and use of chitosan-GSNO nanoparticles for improving drought stress tolerance in soybean. Results: The CSGSNONPs released NO gas for a significantly longer period and at a much lower rate as compared to free GSNO indicating that incorporation of GSNO in CSNPs can protect the NO-donor from rapid decomposition and ensure optimal NO release. CS-GSNONPs improved drought tolerance in soybean plants reflected by a significant increase in plant height, biomass, root length, root volume, root surface area, number of root tips, forks, and nodules. Further analyses indicated significantly lower electrolyte leakage, higher proline content, higher catalase, and ascorbate peroxidase activity, and reduction in MDA and H2O2 contents after treatment with 50 μM CS-GSNONPs under drought stress conditions. Quantitative real-time PCR analysis indicated that CS-GSNONPs protected against drought-induced stress by regulating the expression of drought stress-related marker genes such as GmDREB1a, GmP5CS, GmDEFENSIN, and NO-related genes GmGSNOR1 and GmNOX1. Conclusions: This study highlights the potential of nano-technology-based delivery systems for nitric oxide donors to improve plant growth, and development and protect against stresses.

KW - Chitosan

KW - Nitric oxide

KW - GSNO

KW - Glycine max

KW - Nanoparticles

U2 - 10.1186/s12870-023-04640-x

DO - 10.1186/s12870-023-04640-x

M3 - Article

SN - 1471-2229

VL - 23

JO - BMC Plant Biology

JF - BMC Plant Biology

M1 - 639

ER -

Chitosan-GSNO nanoparticles: a positive modulator of drought stress tolerance in soybean

Abstract

Keywords

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