Charting the cellular and extracellular proteome analysis of Brevibacterium linens DSM 20158 with unsequenced genome by mass spectrometry-driven sequence similarity searches

Khadija Shabbiri, Catherine Helen Botting, Ahmad Adnan, Matthew Alexander Fuszard

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Brevibacterium linens DSM 20158 is an industrially important actinobacterium which is well-known for the production of amino acids and enzymes. However, as this strain has an unsequenced genome, there is no detailed information regarding its proteome although another strain of this microbe, BL2, has a shotgun genome sequence. However, this still does not cover the entire scope of its proteome. The present study is carried out by first identifying proteins by homology matches using the Mascot search algorithm followed by an advanced approach using de novo sequencing and MS BLAST to expand the B. linens proteome. The proteins identified in the secretome and cellular portion appear to be involved in various metabolic and physiological processes of this unsequenced organism. This study will help to enhance the usability of this strain of B. linens in different areas of research in the future rather than mainly in the food industries.
Original languageEnglish
Pages (from-to)99-118
Number of pages20
JournalJournal of Proteomics
Volume83
DOIs
Publication statusPublished - 27 May 2013

Keywords

  • B. linens
  • De novo sequencing
  • nLC MS/MS
  • Cellular proteome
  • Actinobacteria Extracellular proteome

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