Characterization of monoclonal antibodies to a U.K. isolate of barley yellow dwarf virus

L. Torrance; M. T. Pead; A. P. Larkins; G. W. Butcher

doi:10.1099/0022-1317-67-3-549

Characterization of monoclonal antibodies to a U.K. isolate of barley yellow dwarf virus

L. Torrance, M. T. Pead, A. P. Larkins, G. W. Butcher

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Abstract

Five rat monoclonal antibodies (MAbs) specific for U.K. isolate B of barley yellow dwarf virus (BYDV) have been produced and tested. The reactions of the MAbs against a range of isolates of known vector specificity from the U.K., U.S.A. and Sweden were compared and a provisional serological correlation suggested. A survey of 90 U.K. field isolates showed that they were of the same serological groupings as the typed isolates, and that the panel of MAbs could be used to characterize field isolates of BYDV. Two of the MAbs were produced in large quantities in ascitic fluids; antibody partially purified from them and conjugated to alkaline phosphatase was used successfully in direct ELISA.

Original language	English
Pages (from-to)	549-556
Number of pages	8
Journal	Journal of General Virology
Volume	67
Issue number	3
DOIs	https://doi.org/10.1099/0022-1317-67-3-549
Publication status	Published - 1 Jan 1986

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abstract = "Five rat monoclonal antibodies (MAbs) specific for U.K. isolate B of barley yellow dwarf virus (BYDV) have been produced and tested. The reactions of the MAbs against a range of isolates of known vector specificity from the U.K., U.S.A. and Sweden were compared and a provisional serological correlation suggested. A survey of 90 U.K. field isolates showed that they were of the same serological groupings as the typed isolates, and that the panel of MAbs could be used to characterize field isolates of BYDV. Two of the MAbs were produced in large quantities in ascitic fluids; antibody partially purified from them and conjugated to alkaline phosphatase was used successfully in direct ELISA.",

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AB - Five rat monoclonal antibodies (MAbs) specific for U.K. isolate B of barley yellow dwarf virus (BYDV) have been produced and tested. The reactions of the MAbs against a range of isolates of known vector specificity from the U.K., U.S.A. and Sweden were compared and a provisional serological correlation suggested. A survey of 90 U.K. field isolates showed that they were of the same serological groupings as the typed isolates, and that the panel of MAbs could be used to characterize field isolates of BYDV. Two of the MAbs were produced in large quantities in ascitic fluids; antibody partially purified from them and conjugated to alkaline phosphatase was used successfully in direct ELISA.

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Characterization of monoclonal antibodies to a U.K. isolate of barley yellow dwarf virus

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