Characterization of Maguari orthobunyavirus mutants suggests the nonstructural protein NSm is not essential for growth in tissue culture.

E Pollitt, J Zhao, P Muscatt, Richard Michael Elliott

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Maguari virus (MAGV; genus Orthobunyavirus, family Bunyaviridae) contains a tripartite negative-sense RNA genome. Like all orthobunyaviruses, the medium (M) genome segment encodes a precursor polyprotein (NH2-Gn-NSm-Gc-COOH) for the two virion glycoproteins Gn and Cc and a nonstructural protein NSm. The nucleotide sequences of the M segment of wild-type (wt) MAGV, of a temperature-sensitive (ts) mutant, and of two non-ts revertants, R1 and R2, that show electrophoretic mobility differences in their Gc proteins were determined. Twelve amino acid differences (2 in Gn, 10 in Gc) were observed between wt and ts MAGV, of which 9 were maintained in R1 and R2. The M RNA segments of R1 and R2 contained internal deletions, resulting in the removal of the N-terminal 239 residues of Gc (R1) or the C-terminal two thirds of NSm and the N-terminal 431 amino acids of Gc (R2). The sequence data were consistent with analyses of the virion RNAs and virion glycoproteins. These results suggest that neither the N-terminal domain of Gc nor an intact NSm protein is required for the replication of MAGV in tissue culture. (c) 2005 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)224-232
Number of pages9
JournalVirology
Volume348
Issue number1
DOIs
Publication statusPublished - 25 Apr 2006

Keywords

  • bunyavirus
  • Maguari virus
  • nonstructural protein
  • glycoprotein
  • DEFECTIVE INTERFERING PARTICLES
  • CALIFORNIA ENCEPHALITIS-VIRUS
  • CACHE VALLEY VIRUS
  • MEDIUM RNA SEGMENT
  • BUNYAMWERA-VIRUS
  • SEROGROUP VIRUSES
  • NUCLEOTIDE-SEQUENCE
  • GENE-PRODUCT
  • IN-VIVO
  • BUNYAVIRUS

Fingerprint

Dive into the research topics of 'Characterization of Maguari orthobunyavirus mutants suggests the nonstructural protein NSm is not essential for growth in tissue culture.'. Together they form a unique fingerprint.

Cite this