Characterization of Lactobacillus salivarius CECT 5713, a strain isolated from human milk: from genotype to phenotype

Susana Langa, Antonio Maldonado-Barragan, Susana Delgado, Rebeca Martin, Virginia Martin, Esther Jimenez, Jose L. Ruiz-Barba, Baltasar Mayo, Ruth I. Connor, Juan Evaristo Suarez, Juan M. Rodriguez*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

CECT 5713, isolated from human milk, has immunomodulatory, anti-inflammatory and antiinfectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, the relationships between several genetic features of CECT 5713 and the corresponding phenotypes were evaluated. Although it contains a plasmid-encoded bacteriocin cluster, no bacteriocin biosynthesis was observed, possibly due to a 4-bp deletion at the beginning of the histidine kinase determinant . The genome of CECT 5713 harbours two apparently complete prophages of 39.6 and 48 kbp. Upon induction, the 48-kbp prophage became liberated from the bacterial genome, but no DNA replication took place, which resulted in lysis of the cultures but not in phage progeny generation. The strain was sensitive to most antibiotics tested and no transmissible genes potentially involved in antibiotic resistance were detected. Finally, the genome of CECT 5713 contained four ORFs potentially involved in human molecular mimetism. Among them, protein 1230 was considered of particular relevance because of its similarity with dendritic cell-related proteins. Subsequently, in vitro assays revealed the ability of CECT 5713 to stimulate the maturation of immature dendritic cells and to inhibit the in vitro infectivity of HIV-1.

Original languageEnglish
Pages (from-to)1279-1287
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume94
Issue number5
DOIs
Publication statusPublished - Jun 2012

Keywords

  • Lactobacillus salivarius
  • Probiotic
  • Genome
  • Phage
  • Antibiotic resistance
  • Bacteriocin
  • Dendritic cell
  • HIV
  • LACTIC-ACID BACTERIA
  • DENDRITIC CELLS
  • BREAST-MILK
  • ANTIBIOTIC SUSCEPTIBILITY
  • INFECTIOUS MASTITIS
  • GENOME SEQUENCE
  • INFANT FECES
  • BIFIDOBACTERIA
  • TRANSMISSION
  • SALMONELLA

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