Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus xylophilus

Margarida Espada, John T. Jones, Manuel Mota

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We have previously identified two secreted glutathione S-transferases (GST) expressed in the pharyngeal gland cell of Bursaphelenchus xylophilus, which are upregulated post infection of the host. This study examines the functional role of GSTs in B. xylophilus biology. We analysed the expression profiles of all predicted GSTs in the genome and the results showed that they belong to kappa and cytosolic subfamilies and the majority are upregulated post infection of the host. A small percentage is potentially secreted and none is downregulated post infection of the host. One secreted protein was confirmed as a functional GST and is within a cluster that showed the highest expression fold change in infection. This enzyme has a protective activity that may involve host defences, namely in the presence of terpenoid compounds and peroxide products. These results suggest that GSTs secreted into the host participate in the detoxification of host-derived defence compounds and enable successful parasitism.
Original languageEnglish
Pages (from-to)697-709
Number of pages13
JournalNematology
Volume18
Issue number6
Early online date10 May 2016
DOIs
Publication statusPublished - 2016

Keywords

  • Detoxification metabolism
  • Effectors
  • Plant-parasitic nematode
  • Terpenoid compounds

Fingerprint

Dive into the research topics of 'Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus xylophilus'. Together they form a unique fingerprint.

Cite this