Characteristics of L-carnitine transport by lactating rat mammary tissue

DB Shennan, Rona Ruth Ramsay, A Grant, C Burns, VA Zammit

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The transport of L-carnitine by lactating rat mammary tissue has been examined. L-Carnitine uptake by rat mammary tissue explants isolated from lactating rats, 3-4 days post partum, was via both Na+-dependent and Na+-independent pathways. The Na+-dependent pathway, the predominant route for L-carnitine uptake, was a saturable process: the K-m and V-max were, respectively, 132 mu M and 201 pmol/2 h/mg of intracellular water. The Na+-independent pathway, which was nonsaturable, had a coefficient of 0.26 mu l/mg of intracellular water/2 h. The Na+-dependent component of L-carnitine uptake by mammary tissue explants was cis-inhibited by D-carnitine and acetyl-L-carnitine, but not by choline or taurine. In contrast, the Na+-independent component of L-carnitine uptake was not affected by any of these compounds. The uptake of L-carnitine by mammary tissue isolated from lactating rats, 10-12 days post partum, was qualitatively similar to that by mammary tissue taken from rats during the early stage of lactation. However, L-carnitine uptake was quantitatively lower: this was attributable to a reduction in the Na+-dependent component of L-carnitine uptake. L-Carnitine efflux from rat mammary tissue taken from animals 3-4 days post partum, consisted of at least two components; a fast extracellular component and a slow membrane-limited component. Reversing the trans-membrane Na+-gradient did not stimulate L-carnitine afflux suggesting that the Na+-dependent L-carnitine carrier operates with asymmetrical kinetics. A hyposmotic shock, hence cell-swelling, increased L-carnitine efflux from mammary tissue explants. (C) 1998 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalBiochimica et Biophysica Acta
Volume1393
Publication statusPublished - 31 Jul 1998

Keywords

  • mammary gland
  • L-carnitine
  • sodium dependence
  • BORDER-MEMBRANE-VESICLES
  • ANION-EXCHANGE
  • MECHANISM
  • CELLS
  • SPECIFICITY
  • COTRANSPORT
  • SECRETION
  • TAURINE
  • MILK

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