Characterisation of barley resistance to rhynchosporium on chromosome 6HS

Max Coulter; Bianca Buettner; Kerstin Hofmann; Micha Bayer; Luke Ramsay; Guenther Schweizer; Robbie Waugh; Mark E. Looseley; Anna Avrova

doi:10.1007/s00122-018-3262-8

Characterisation of barley resistance to rhynchosporium on chromosome 6HS

Max Coulter, Bianca Buettner, Kerstin Hofmann, Micha Bayer, Luke Ramsay, Guenther Schweizer, Robbie Waugh, Mark E. Looseley, Anna Avrova^*

^*Corresponding author for this work

School of Biology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S₂ and S₃ lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.

Original language	English
Pages (from-to)	1089-1107
Number of pages	19
Journal	Theoretical and Applied Genetics
Volume	132
Issue number	4
Early online date	13 Dec 2018
DOIs	https://doi.org/10.1007/s00122-018-3262-8
Publication status	Published - Apr 2019

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https://discovery.dundee.ac.uk/en/publications/characterisation-of-barley-resistance-to-rhynchosporium-on-chromo

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@article{89af77d4014e458f8ff73e4443c412e6,

title = "Characterisation of barley resistance to rhynchosporium on chromosome 6HS",

abstract = "Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S2 and S3 lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.",

author = "Max Coulter and Bianca Buettner and Kerstin Hofmann and Micha Bayer and Luke Ramsay and Guenther Schweizer and Robbie Waugh and Looseley, {Mark E.} and Anna Avrova",

note = "This work was funded by the Scottish Food Security Alliance (SFSA) and the Bavarian State Ministry of Food, Agriculture and Forestry. MB, LR, RW, MEL and AA were supported by the Scottish Government Rural and Environment Science and Analytical Services (RESAS). AA and MB were also funded by the BBSRC-CIRC Project BB/J019569/1. KH was supported by the Federal Office of Agriculture and Food (BLE) under Grant No 28-1-41.009-06.",

year = "2019",

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doi = "10.1007/s00122-018-3262-8",

language = "English",

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pages = "1089--1107",

journal = "Theoretical and Applied Genetics",

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T1 - Characterisation of barley resistance to rhynchosporium on chromosome 6HS

AU - Coulter, Max

AU - Buettner, Bianca

AU - Hofmann, Kerstin

AU - Bayer, Micha

AU - Ramsay, Luke

AU - Schweizer, Guenther

AU - Waugh, Robbie

AU - Looseley, Mark E.

AU - Avrova, Anna

N1 - This work was funded by the Scottish Food Security Alliance (SFSA) and the Bavarian State Ministry of Food, Agriculture and Forestry. MB, LR, RW, MEL and AA were supported by the Scottish Government Rural and Environment Science and Analytical Services (RESAS). AA and MB were also funded by the BBSRC-CIRC Project BB/J019569/1. KH was supported by the Federal Office of Agriculture and Food (BLE) under Grant No 28-1-41.009-06.

PY - 2019/4

Y1 - 2019/4

N2 - Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S2 and S3 lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.

AB - Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S2 and S3 lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.

U2 - 10.1007/s00122-018-3262-8

DO - 10.1007/s00122-018-3262-8

M3 - Article

SN - 0040-5752

VL - 132

SP - 1089

EP - 1107

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

IS - 4

ER -

Characterisation of barley resistance to rhynchosporium on chromosome 6HS

Abstract

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