Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet

Aleksandra Jovanovic; Milan Obradovic; Emina Sudar-Milovanovic; Alan J. Stewart; Samantha J. Pitt; Dragan Alvantic; Ema Aleksic; Esma R. Isenovic

doi:10.1007/s11010-017-3077-y

Changes in cardiac Na⁺/K⁺-ATPase expression and activity in female rats fed a high fat diet

Aleksandra Jovanovic, Milan Obradovic, Emina Sudar-Milovanovic, Alan J. Stewart, Samantha J. Pitt, Dragan Alvantic, Ema Aleksic, Esma R. Isenovic

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na⁺/K⁺-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na⁺/K⁺-ATPase by 30% (p < 0.05), expression of total α₁ subunit of Na⁺/K⁺-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na⁺/K⁺-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.

Original language	English
Pages (from-to)	49-58
Number of pages	10
Journal	Molecular and Cellular Biochemistry
Volume	436
Issue number	1-2
Early online date	31 May 2017
DOIs	https://doi.org/10.1007/s11010-017-3077-y
Publication status	Published - Dec 2017

Keywords

High-fat diet
Estradiol
NA+/K+-ATPase
Obesity
RhoA/ROCK signalling
Female

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10.1007/s11010-017-3077-y

Jovanovic-2017-Changes-in-cardiac-MCB-AAM
© 2017, Springer. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at link.springer.com / https://doi.org/10.1007/s11010-017-3077-y
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Cite this

@article{fa5e03239ffe4f5b8906b2fe51ca20f6,

title = "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet",

abstract = "The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.",

keywords = "High-fat diet, Estradiol, NA+/K+-ATPase, Obesity, RhoA/ROCK signalling, Female",

author = "Aleksandra Jovanovic and Milan Obradovic and Emina Sudar-Milovanovic and Stewart, {Alan J.} and Pitt, {Samantha J.} and Dragan Alvantic and Ema Aleksic and Isenovic, {Esma R.}",

note = "This work is supported by the Grant Nos. 173033 and III41028 from the Ministry of Science, Republic of Serbia.",

year = "2017",

month = dec,

doi = "10.1007/s11010-017-3077-y",

language = "English",

volume = "436",

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journal = "Molecular and Cellular Biochemistry",

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TY - JOUR

T1 - Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet

AU - Jovanovic, Aleksandra

AU - Obradovic, Milan

AU - Sudar-Milovanovic, Emina

AU - Stewart, Alan J.

AU - Pitt, Samantha J.

AU - Alvantic, Dragan

AU - Aleksic, Ema

AU - Isenovic, Esma R.

N1 - This work is supported by the Grant Nos. 173033 and III41028 from the Ministry of Science, Republic of Serbia.

PY - 2017/12

Y1 - 2017/12

N2 - The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.

AB - The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.

KW - High-fat diet

KW - Estradiol

KW - NA+/K+-ATPase

KW - Obesity

KW - RhoA/ROCK signalling

KW - Female

U2 - 10.1007/s11010-017-3077-y

DO - 10.1007/s11010-017-3077-y

M3 - Article

SN - 0300-8177

VL - 436

SP - 49

EP - 58

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

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ER -