Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata

Zoe Barr; Jens Tilsner

doi:10.1007/978-1-0716-2867-6_14

Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata

Zoe Barr, Jens Tilsner^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The actin cytoskeleton has close but so far incompletely understood connections to plasmodesmata, the cell junctions of plants. Plasmodesmata are essential for plant development and responses to biotic and abiotic stresses and facilitate the intercellular exchange of metabolites and hormones but also macromolecules such as proteins and RNAs. The molecular size exclusion limited of plasmodesmata is dynamically regulated, including by actin-associated proteins. Therefore, experimental analysis of plasmodesmal regulation can be relevant to plant cytoskeleton research. This chapter presents two simple imaging-based protocols for analyzing macromolecular cell-to-cell connectivity in leaves.

Original language	English
Title of host publication	The plant cytoskeleton
Subtitle of host publication	methods and protocols
Editors	Patrick J. Hussey, Pengwei Wang
Place of Publication	New York, NY
Publisher	Humana Press
Chapter	14
Pages	193-202
Number of pages	10
ISBN (Electronic)	9781071628676
ISBN (Print)	9781071628669, 9781071628690
DOIs	https://doi.org/10.1007/978-1-0716-2867-6_14
Publication status	Published - 12 Feb 2023

Publication series

Name	Methods in molecular biology
Volume	2604
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-0716-2867-6_14

Cytoskeleton_PD_methods_chapter_AAM
Copyright © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1007/978-1-0716-2867-6_14.
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Cite this

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abstract = "The actin cytoskeleton has close but so far incompletely understood connections to plasmodesmata, the cell junctions of plants. Plasmodesmata are essential for plant development and responses to biotic and abiotic stresses and facilitate the intercellular exchange of metabolites and hormones but also macromolecules such as proteins and RNAs. The molecular size exclusion limited of plasmodesmata is dynamically regulated, including by actin-associated proteins. Therefore, experimental analysis of plasmodesmal regulation can be relevant to plant cytoskeleton research. This chapter presents two simple imaging-based protocols for analyzing macromolecular cell-to-cell connectivity in leaves.",

author = "Zoe Barr and Jens Tilsner",

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Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata. / Barr, Zoe; Tilsner, Jens.
The plant cytoskeleton: methods and protocols. ed. / Patrick J. Hussey; Pengwei Wang. New York, NY: Humana Press, 2023. p. 193-202 (Methods in molecular biology; Vol. 2604).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata

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