Carotenoid production in Lactobacillus plantarum

Juan Garrido-Fernandez, Antonio Maldonado-Barragan, Belen Caballero-Guerrero, Damaso Hornero-Mendez, Jose Luis Ruiz-Barba*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Eighteen strains of Lactobacillus plantarum from different origins were screened for carotenoid production, as many of them exhibited a deep yellow pigmentation when cultured as isolated colonies on MRS-agar plates. We found that most of them produced significative amounts of the yellow C-30 carotenoid 4,4'-diaponeurosporene in the range 1.8 to 54 mg/kg of dry cell weight. Although some of the strains produced just trace amounts of this carotenoid, PCR studies showed that all of them harbored the genes crtM and crtN which, inferred from homology, had been predicted in the three L. plantarum complete genome sequences currently available. Our results suggest the full functionality of a C-30 carotenoid biosynthesis pathway in this species, driven by the operon crtNM. DNA sequencing of the entire crtNM operon in the maximum carotenoid-producing strain found in this study, i.e. L plantarum CECT7531, was accomplished. Genes crtM and crtN were annotated as dehydrosqualene synthase and dehydrosqualene desaturase, respectively, in this strain. (C) 2010 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalInternational Journal of Food Microbiology
Volume140
Issue number1
DOIs
Publication statusPublished - 30 May 2010

Keywords

  • Lactobacillus plantarum
  • Carotenoid
  • Diaponeurosporene
  • Dehydrosqualene
  • Food fermentations
  • COMPLETE GENOME SEQUENCE
  • LACTIC-ACID BACTERIA
  • STAPHYLOCOCCUS-AUREUS
  • TRITERPENOID CAROTENOIDS
  • PROTECTIVE CULTURES
  • C-30
  • BIOSYNTHESIS
  • PIGMENTS
  • STRAINS
  • PATHWAY

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