cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6

A N Smith; C J Wardle; J P Winpenny; B Verdon; M A Gray; B E Argent; A Harris

doi:10.1016/0925-4439(95)00047-8

cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6

A N Smith, C J Wardle, J P Winpenny, B Verdon, M A Gray, B E Argent, A Harris

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.

Original language	English
Pages (from-to)	315-20
Number of pages	6
Journal	Biochimica et Biophysica Acta
Volume	1271
Issue number	2-3
DOIs	https://doi.org/10.1016/0925-4439(95)00047-8
Publication status	Published - 9 Jun 1995

Keywords

Adenocarcinoma/genetics
Cell Line
Chloride Channels/chemistry
Cyclic AMP/pharmacology
Cystic Fibrosis Transmembrane Conductance Regulator
Genetic Vectors
Membrane Proteins/biosynthesis
Pancreatic Neoplasms/genetics
Patch-Clamp Techniques
RNA, Messenger/analysis
Transfection
Tumor Cells, Cultured

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10.1016/0925-4439(95)00047-8

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title = "cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6",

abstract = "Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.",

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author = "Smith, {A N} and Wardle, {C J} and Winpenny, {J P} and B Verdon and Gray, {M A} and Argent, {B E} and A Harris",

year = "1995",

month = jun,

day = "9",

doi = "10.1016/0925-4439(95)00047-8",

language = "English",

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T1 - cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6

AU - Smith, A N

AU - Wardle, C J

AU - Winpenny, J P

AU - Verdon, B

AU - Gray, M A

AU - Argent, B E

AU - Harris, A

PY - 1995/6/9

Y1 - 1995/6/9

N2 - Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.

AB - Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.

KW - Adenocarcinoma/genetics

KW - Cell Line

KW - Chloride Channels/chemistry

KW - Cyclic AMP/pharmacology

KW - Cystic Fibrosis Transmembrane Conductance Regulator

KW - Genetic Vectors

KW - Membrane Proteins/biosynthesis

KW - Pancreatic Neoplasms/genetics

KW - Patch-Clamp Techniques

KW - RNA, Messenger/analysis

KW - Transfection

KW - Tumor Cells, Cultured

U2 - 10.1016/0925-4439(95)00047-8

DO - 10.1016/0925-4439(95)00047-8

M3 - Article

C2 - 7541649

SN - 0006-3002

VL - 1271

SP - 315

EP - 320

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

IS - 2-3

ER -

cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6

Abstract

Keywords

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