Abstract
N1E-115 mouse neuroblastoma cells were injected with a calcium buffer/indicator solution to allow both ratiometric measurement of free calcium concentration and the release of calcium ions upon UV flash. The solution contained sulforhodamine, a marker dye used to estimate the volume injected; fluo-3, a calcium indicator, and NP-EGTA, a high affinity calcium-selective buffer that is converted by UV hash to products with negligible calcium affinity. The calcium increase recorded upon UV irradiation (Delta[Ca2+](i)) was small for small injection volumes, increased with larger injection volumes, but approached a plateau at the largest injection volumes. From this relation we estimate the buffering capacity of the cytosol as 1700 ions bound perion free. (C) 1998 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 786-790 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 250 |
| Issue number | 3 |
| Publication status | Published - 29 Sept 1998 |
Keywords
- calcium homeostasis
- calcium buffering
- flash photolysis
- nerve cell
- INTRACELLULAR CALCIUM
- BLASTOMA CELLS
- RAT
- CA2+