Calcium binding proteins in motoneurons at low and high risk for degeneration in ALS

P Laslo, J Lipski, L F B Nicholson, Gareth Brian Miles, G D Funk

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Recent reports challenge the hypothesis that expression of calcium binding proteins contributes to the greater resistance of some motoneurons to degeneration in amyotrophic lateral sclerosis (ALS). We therefore re-examined, using immunohistochemistry, the expression of calbindin, calretinin and parvalbumin in vulnerable (hypoglossal, XII; and cervical spinal) and resistant (oculomotor, III) motoneurons of adult rats. Calbindin immunoreactivity was lacking in motor nuclei but strong in the dorsal horn. Calretinin was expressed in spinal, but not III or XII, motoneurons. Parvalbumin immunoreactivity, tested with a polyclonal antibody, was intense in spinal and ill, but not XII, motoneurons; however, no staining in the ventral horn was observed with a monoclonal antibody. Differential expression of calretinin and parvalbumin within vulnerable motoneurons suggests that immunoreactivity for these proteins is not a reliable marker for resistance to degeneration in ALS. NeuroReport 11:3305-3308 (C) 2000 Lippincott Williams & Wilkins.

Original languageEnglish
Pages (from-to)3305-3308
Number of pages4
JournalNeuroreport
Volume11
Issue number15
DOIs
Publication statusPublished - 20 Oct 2000

Keywords

  • calbindin
  • calretinin
  • hypoglossal
  • immunocytochemistry
  • neurodegeneration
  • oculomotor
  • parvalbumin
  • rat
  • ventral horn
  • AMYOTROPHIC-LATERAL-SCLEROSIS
  • MOTOR-NEURON DISEASE
  • RAT SPINAL-CORD
  • NERVOUS-SYSTEM
  • PARVALBUMIN
  • EXPRESSION
  • CALRETININ
  • CALBINDIN-D-28K
  • MOUSE
  • BRAIN

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