TY - JOUR
T1 - Calcineurin subunit B is involved in shell regeneration in Haliotis diversicolor
AU - Buddawong, Tiranan
AU - Asuvapongpatana, Somluk
AU - Suwannasing, Chanyatip
AU - Habuddha, Valainipha
AU - Sukonset, Chompoonut
AU - Sombutkayasith, Chanyarak
AU - McDougall, Carmel
AU - Weerachatyanukul, Wattana
N1 - Funding: This study was supported by the Thailand Research Fund (TRF) through a Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0047/2554).
PY - 2021/1/12
Y1 - 2021/1/12
N2 - Abalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.
AB - Abalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.
KW - Shell
KW - Regeneration
KW - Haliotis diversicolor
KW - Biomineralization
KW - Calcineurin
U2 - 10.7717/peerj.10662
DO - 10.7717/peerj.10662
M3 - Article
C2 - 33520456
SN - 2167-8359
VL - 9
JO - PeerJ
JF - PeerJ
M1 - e10662
ER -