Cajal body proteins SMN and coilin show differential dynamic behaviour in vivo

Judith Elizabeth Sleeman, L Trinkle-Mulcahy, AR Prescott, SC Ogg, AI Lamond

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)

Abstract

Analysis of stable cell lines expressing fluorescently tagged survival of motor neurons protein (SMN) and coilin shows striking differences in their dynamic behaviour, both in the nucleus and during mitosis. Cajal bodies labelled with either FP-SMN or FP-coilin show similar behaviour and frequency of movements. However, fluorescence recovery after photobleaching (FRAP) studies show that SMN returns similar to50-fold more slowly to Cajal bodies than does coilin. Time-lapse studies on cells progressing from prophase through to G1 show further differences between SMN and coilin, both in their localisation in telophase and in the timing of their re-entry into daughter nuclei. The data reveal similarities between Cajal bodies and nucleoli in their behaviour during mitosis. This in vivo study indicates that SMN and coilin interact differentially with Cajal bodies and reveals parallels in the pathway for reassembly of nucleoli and Cajal bodies following mitosis.

Original languageEnglish
Pages (from-to)2039-2050
Number of pages12
JournalJournal of Cell Science
Volume116
Issue number10
DOIs
Publication statusPublished - 15 May 2003

Keywords

  • SMN
  • coilin
  • nucleus
  • cajal bodies
  • nucleolus
  • mitosis
  • SPINAL MUSCULAR-ATROPHY
  • SMALL NUCLEAR RIBONUCLEOPROTEINS
  • GREEN FLUORESCENT PROTEIN
  • DISEASE GENE-PRODUCT
  • SPLICING SNRNPS
  • SPLICEOSOMAL SNRNPS
  • MUTATIONAL ANALYSIS
  • ASSEMBLY PATHWAY
  • CDNA CLONING
  • HUMAN-CELLS

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